Li Yan, Wo John M, Ellis Susan, Ray Mukunda B, Jones Whitney, Martin Robert C
Division of Surgical Oncology, University of Louisville School of Medicine, Louisville, Kentucky 40202, USA.
Dig Dis Sci. 2006 Mar;51(3):527-32. doi: 10.1007/s10620-006-3165-4.
The current animal models of esophagitis and Barrett's esophagus consist of surgeries that divert the gastroduodenal contents to the esophagus. The limitations of these models are the inability to control the amount and concentration of the refluxate and the causing of significant postoperative stress and morbidity. Eighteen adult rats were cannulated at the upper esophagus and connected to a subcutaneous osmotic micropump to perfuse the esophageal lumen with bile and acid. Animals were sacrificed after 7 days of perfusion. Histological changes were determined. Cell proliferation, apoptosis, lipid peroxidation, and glutathione were measured. Histopathological changes in the bile- or acid-perfused esophagus were consistent with the findings associated with reflux esophagitis. Enhanced proliferation and apoptosis were seen, along with increased oxidative stress. The external esophageal perfusion model enabled precise control of the injurious agent. It induced the histologic and cellular injury of reflux esophagitis after 7 days.
目前的食管炎和巴雷特食管动物模型包括将胃十二指肠内容物转流至食管的手术。这些模型的局限性在于无法控制反流物的量和浓度，以及会导致显著的术后应激和发病率。18只成年大鼠在上段食管插管，并连接到皮下渗透微型泵，以便用胆汁和酸灌注食管腔。灌注7天后处死动物。确定组织学变化。测量细胞增殖、凋亡、脂质过氧化和谷胱甘肽。胆汁或酸灌注食管的组织病理学变化与反流性食管炎的相关发现一致。观察到增殖和凋亡增强，同时氧化应激增加。食管外部灌注模型能够精确控制损伤因子。7天后它诱发了反流性食管炎的组织学和细胞损伤。
