Zhang Zheng, Emanetoglu Nuri William, Saraf Gaurav, Chen Yimin, Wu Pan, Zhong Jian, Lu Yicheng, Chen Jingqiu, Mirochnitchenko Oleg, Inouye Masayori
Department of Electrical and Computer Engineering, Rutgers University, Piscataway, NJ 08854-8058, USA.
IEEE Trans Ultrason Ferroelectr Freq Control. 2006 Apr;53(4):786-92.
DNA immobilization enhancement is demonstrated in a structure consisting of ZnO nanotips on 128 degrees Y-cut LiNbO3. The ZnO nanotips are grown by metalorganic chemical vapor deposition (MOCVD) on the top of a SiO2 layer that is deposited and patterned on the LiNbO3 SAW delay path. The effects of ZnO nanotips on the SAW response are investigated. X-ray diffraction and scanning electron microscopy are used to analyze the ZnO nanotips, which are of single crystalline quality, and they are uniformly aligned with their c-axis perpendicular to the substrate surface. The photoluminescence (PL) spectrum of the ZnO nanotips shows strong near bandedge transition with insignificant deep level emission, confirming their good optical property. DNA immobilization enhancement is experimentally validated by radioactive labeling tests and SAW response changes. The ZnO nanotips enhance the DNA immobilization by a factor of 200 compared to ZnO film with flat surface. DNA hybridization with complementary and noncomplementary second strand DNA oligonucleotides is used to study the selective binding of the structure. This device structure possesses the advantages of both traditional SAW sensors and ZnO nanostructures.
在由128°Y切割的LiNbO₃上的ZnO纳米尖端组成的结构中，证明了DNA固定化增强。ZnO纳米尖端通过金属有机化学气相沉积（MOCVD）生长在SiO₂层的顶部，该SiO₂层沉积并图案化在LiNbO₃表面声波延迟路径上。研究了ZnO纳米尖端对表面声波响应的影响。使用X射线衍射和扫描电子显微镜分析ZnO纳米尖端，其具有单晶质量，并且它们的c轴垂直于衬底表面均匀排列。ZnO纳米尖端的光致发光（PL）光谱显示出强烈的近带边跃迁，深能级发射不明显，证实了它们良好的光学性质。通过放射性标记测试和表面声波响应变化对DNA固定化增强进行了实验验证。与具有平坦表面的ZnO薄膜相比，ZnO纳米尖端将DNA固定化提高了200倍。使用与互补和非互补第二链DNA寡核苷酸的DNA杂交来研究该结构的选择性结合。这种器件结构具有传统表面声波传感器和ZnO纳米结构的优点。
