Goufman E I, Moshkovskii S A, Tikhonova O V, Lokhov P G, Zgoda V G, Serebryakova M V, Toropygin I Yu, Vlasova M A, Safarova M R, Makarov O V, Archakov A I
Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, 119121 Moscow, Russia.
Biochemistry (Mosc). 2006 Apr;71(4):354-60. doi: 10.1134/s000629790604002x.
One of the problems of plasma proteomics is a presence of large major components. In this work, we use the thermostable fraction as a way to deplete these major proteins. The thermostable fraction of serum samples from patients with ovarian, uterus, and breast cancers and benign ovarian tumor was analyzed using two-dimensional electrophoresis combined with MALDI-TOF(-TOF)-mass spectrometry. Of them, alpha-1-acid glycoprotein and clusterin are expressly down-regulated in breast cancer, whereas transthyretin is decreased specifically in ovarian cancer. Apolipoprotein A-I forms have decreased spot volumes, while haptoglobin alpha1, in contrast, is elevated in several tumors. These data are partly consistent with previous art studies on cancer proteomics, which involve mass-spectrometry-based serum profiling techniques. Serum thermostable fraction may be recommended as a good tool for medium and small protein proteome investigation, in particular, by 2D-electrophoresis.
血浆蛋白质组学的问题之一是存在大量主要成分。在这项工作中，我们使用热稳定组分作为去除这些主要蛋白质的一种方法。采用二维电泳结合基质辅助激光解吸电离飞行时间（-串联飞行时间）质谱分析卵巢癌、子宫癌、乳腺癌患者及卵巢良性肿瘤患者血清样本的热稳定组分。其中，α-1-酸性糖蛋白和簇集蛋白在乳腺癌中明显下调，而转甲状腺素蛋白在卵巢癌中特异性降低。载脂蛋白A-I各亚型的斑点体积减小，而触珠蛋白α1在几种肿瘤中升高。这些数据与之前基于质谱的血清谱技术的癌症蛋白质组学研究部分一致。血清热稳定组分可推荐作为中、小蛋白质蛋白质组研究的良好工具，特别是通过二维电泳。
