Yurina N P, Pogulskaya E N, Karapetyan N V
Bach Institute of Biochemistry, Russian Academy of Sciences, 119071 Moscow, Russia.
Biochemistry (Mosc). 2006 Apr;71(4):430-6. doi: 10.1134/s0006297906040110.
The effects of photodestruction of chloroplasts in norflurazon-treated barley seedlings on expression of nuclear genes Elip and Hsp32 encoding light and heat stress proteins of barley chloroplasts and also of the Lhcb1 and RbcS genes of photosynthesis proteins were studied. The genes of the photosynthesis proteins were not transcribed upon the photodestruction of chloroplasts. However, transcription of the stress protein genes continued, and the transcription of the heat stress protein gene remained virtually at the control level, whereas the light stress protein gene transcription was markedly (by 30-50%) decreased, and this suggests chloroplast control of the Elip gene transcription. Disorders in the processing and a partial disturbance in the import of precursors of Hsp32 and Elip proteins into the plastids of the norflurazon-treated seedlings were shown. Data on protease analysis indicates that photodestruction of chloroplasts is associated with accumulation of stress protein precursors in the plastid envelope.
研究了去草净处理的大麦幼苗中叶绿体光破坏对编码大麦叶绿体光应激蛋白和热应激蛋白的核基因Elip和Hsp32以及光合作用蛋白的Lhcb1和RbcS基因表达的影响。叶绿体光破坏后，光合作用蛋白基因不转录。然而，应激蛋白基因的转录仍在继续，热应激蛋白基因的转录几乎维持在对照水平，而光应激蛋白基因的转录明显下降（30%-50%），这表明叶绿体对Elip基因转录有调控作用。结果表明，去草净处理的幼苗中Hsp32和Elip蛋白前体在加工过程中出现紊乱，向质体的转运也受到部分干扰。蛋白酶分析数据表明，叶绿体的光破坏与应激蛋白前体在质体包膜中的积累有关。
