Grigsby Peta L, Sooranna Suren R, Brockman Diane E, Johnson Mark R, Myatt Leslie
Department of Obstetrics and Gynceology, University of Cincinnati, Medical College, Cincinnati, OH 45267, USA.
Am J Obstet Gynecol. 2006 Jul;195(1):260-9. doi: 10.1016/j.ajog.2006.01.082. Epub 2006 Apr 17.
The effects of prostaglandin E2 (PGE2) are mediated through G-protein coupled receptors, acting via different second messengers. The aim of this study was to characterize the temporal and tissue specific localization and expression of the PGE2 receptor subtypes (EP1-4) in uteroplacental tissues during human pregnancy.
Placenta and fetal membranes were collected after delivery at preterm or term, each with or without labor. The localization and expression of the PGE2 receptor subtypes were determined by immunohistochemistry and Western blot.
All 4 receptors were expressed in the placenta and fetal membranes; only EP3 was present in the syncytiotrophoblast layer. EP1 to EP4 were consistently expressed across gestation in the fetal membranes; however, a different cellular localization with labor was observed in the amnion for EP1, EP2, and EP4.
The presence of these receptors in the placenta and fetal membranes may indicate autocrine roles for PGE2 in the signaling pathways associated with placental function and parturition.
前列腺素E2（PGE2）的作用是通过G蛋白偶联受体介导的，经由不同的第二信使发挥作用。本研究的目的是明确人类妊娠期间子宫胎盘组织中PGE2受体亚型（EP1 - 4）的时间和组织特异性定位及表达情况。
在早产或足月分娩后收集胎盘和胎膜，分娩有无发动均可。通过免疫组织化学和蛋白质印迹法确定PGE2受体亚型的定位和表达。
所有4种受体均在胎盘和胎膜中表达；仅EP3存在于合体滋养层。EP1至EP4在胎膜整个妊娠期持续表达；然而，在羊膜中，EP1、EP2和EP4在分娩时细胞定位有所不同。
这些受体在胎盘和胎膜中的存在可能表明PGE2在与胎盘功能和分娩相关的信号通路中具有自分泌作用。
