Kawasaki Makoto, Yamaga Chiyo, Onaka Tatsushi, Saito Jun, Mera Takashi, Hashimoto Hirofumi, Fujihara Hiroaki, Okimoto Nobukazu, Ohnishi Hideo, Nakamura Toshitaka, Ueta Yoichi
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Kitakyushu 807-8555, Japan.
Brain Res. 2006 May 1;1086(1):133-41. doi: 10.1016/j.brainres.2006.02.099. Epub 2006 Apr 17.
The effects of intraperitoneal (i.p.) administration of 2-buten-4-olide (2-B4O), an endogenous sugar acid, on the hypothalamo-neurohypophyseal system were examined in rats. Plasma oxytocin (OXT) levels were significantly increased 15-60 min after i.p. administration of 2-B4O (100 mg/kg), whereas plasma arginine vasopressin (AVP) did not change. Dual immunostaining revealed that Fos-like immunoreactivity (LI) was predominantly observed in OXT-secreting neurons in the paraventricular (PVN) and the supraoptic nuclei (SON) 120 min after i.p. administration of 2-B4O. In addition, many Fos-LI neurons were observed in the nucleus of the tractus solitarius (NTS) after i.p. administration of 2-B4O. These results suggest that a peripherally administered high dose of 2-B4O activates OXT-secreting neurons in the hypothalamus through activation of NTS neurons, possibly as a result of a stress response.
在大鼠中研究了内源性糖酸2-丁烯-4-内酯（2-B4O）腹腔注射（i.p.）对下丘脑-神经垂体系统的影响。腹腔注射2-B4O（100mg/kg）后15 - 60分钟，血浆催产素（OXT）水平显著升高，而血浆精氨酸加压素（AVP）没有变化。双重免疫染色显示，腹腔注射2-B4O 120分钟后，在室旁核（PVN）和视上核（SON）中，Fos样免疫反应性（LI）主要出现在分泌OXT的神经元中。此外，腹腔注射2-B4O后，在孤束核（NTS）中观察到许多Fos-LI神经元。这些结果表明，外周给予高剂量2-B4O可能通过激活NTS神经元来激活下丘脑分泌OXT的神经元，这可能是应激反应的结果。
