Hildebrandt F, Pizzonia J H, Reilly R F, Rebouças N A, Sardet C, Pouysségur J, Slayman C W, Aronson P S, Igarashi P
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510.
Biochim Biophys Acta. 1991 Dec 2;1129(1):105-8. doi: 10.1016/0167-4781(91)90221-7.
Rabbit kidney expresses a transcript that is similar to the human growth-factor-activatable Na+/H+ exchanger. PCR and library screening were used to clone overlapping 2.5 kb, 1.4 kb, and 1.8 kb cDNAs that together contain the entire coding region (2448 bp) and 5' untranslated region (726 bp) and part of the 3' untranslated region (128 bp) of a rabbit renal Na+/H+ exchanger transcript. The nucleotide and inferred amino acid sequences are highly conserved between rabbit and human (88% nucleotide identity, 95% amino acid identity). In rabbit, the transcript is expressed in both epithelial and non-epithelial tissues, with highest expression in stomach, brain, kidney, lung and ileum, and minimal expression in liver and skeletal muscle.
兔肾表达一种与人类生长因子激活的钠氢交换体相似的转录本。采用聚合酶链反应（PCR）和文库筛选技术克隆了重叠的2.5 kb、1.4 kb和1.8 kb互补脱氧核糖核酸（cDNA），它们共同包含兔肾钠氢交换体转录本的完整编码区（2448碱基对）、5'非翻译区（726碱基对）和部分3'非翻译区（128碱基对）。兔与人之间的核苷酸和推导的氨基酸序列高度保守（核苷酸同一性为88%，氨基酸同一性为95%）。在兔中，该转录本在上皮组织和非上皮组织中均有表达，在胃、脑、肾、肺和回肠中表达最高，在肝脏和骨骼肌中表达最低。
