Pelletier Sarah, Lucy Charles A
Department of Chemistry, Gunning/Lemieux Chemistry Centre, University of Alberta, Edmonton, Alta., Canada T6G 2G2.
J Chromatogr A. 2006 Jun 16;1118(1):12-8. doi: 10.1016/j.chroma.2006.03.072. Epub 2006 Apr 17.
Short silica-based monolithic columns (0.5-1 cm) are coated with the surfactant didodecyldimethylammonium bromide (DDAB) and used for fast ion exchange separations of small inorganic anions. Sources of extra-column band broadening are assessed and minimized to obtain separations of seven analytes (iodate, chloride, nitrate, bromide, nitrite, phosphate, sulphate) in two minutes at 2 mL/min. Eluents used are 6 and 9 mM 4-cyanophenol at pH 7.3-7.4 or 5 mM 4-hydroxybenzoic acid at pH 5.6. DDAB coating stability is improved by 15-fold by the addition of a DDAB coated pre-column before the injection valve. Separations are obtained using a low-pressure glass syringe.
短的硅胶整体柱（0.5 - 1厘米）涂有表面活性剂十二烷基二甲基溴化铵（DDAB），用于小无机阴离子的快速离子交换分离。评估并最小化柱外谱带展宽的来源，以便在2毫升/分钟的流速下两分钟内分离七种分析物（碘酸盐、氯化物、硝酸盐、溴化物、亚硝酸盐、磷酸盐、硫酸盐）。使用的洗脱液是pH值为7.3 - 7.4的6和9 mM 4-氰基苯酚或pH值为5.6的5 mM 4-羟基苯甲酸。通过在进样阀前添加一个涂有DDAB的预柱，DDAB涂层稳定性提高了15倍。使用低压玻璃注射器进行分离。
