Confort C, Charrasse S, Clos J
Laboratoire de Neurobiologie Endocrinologie, URA 1197 du CNRS, Université Montpellier II, France.
Neuroreport. 1991 Oct;2(10):566-8. doi: 10.1097/00001756-199110000-00003.
The cerebellar neuroblasts in primary cultures from five-day-old rats bore NGF receptor immunoreactivity, suggesting a potential responsive to NGF. At low plating density, NGF was found to enhance DNA synthesis in these cells in a dose-dependent manner. As these cells synthesize NGF, one possibility to account for the lack of response of neuroblasts plated at high density is that the amount of endogenous trophic agent produced in this culture condition is sufficient to ensure an optimal effect. The results demonstrate that premitotic neuroblasts in the CNS, as well postmitotic neurons, are responsive to NGF. At the early stage of its development, the cerebellum therefore appears to be a very good autocrine model of NGF action.
来自5日龄大鼠的原代培养小脑成神经细胞具有NGF受体免疫反应性，提示其对NGF具有潜在反应性。在低密度接种时，发现NGF以剂量依赖性方式增强这些细胞中的DNA合成。由于这些细胞合成NGF，对于高密度接种的成神经细胞缺乏反应的一种解释可能是，在此培养条件下产生的内源性营养因子的量足以确保最佳效应。结果表明，中枢神经系统中的有丝分裂前成神经细胞以及有丝分裂后神经元均对NGF有反应。因此，在其发育早期，小脑似乎是NGF作用的一个非常好的自分泌模型。
