Jurado E, Fernández-Serrano M, Núñez-Olea J, Luzón G, Lechuga M
Department of Chemical Engineering, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, 18071 Granada, Spain.
Chemosphere. 2006 Oct;65(2):278-85. doi: 10.1016/j.chemosphere.2006.02.044. Epub 2006 Apr 17.
In the present work, we propose a simplified spectrophotometric method for determining anionic surfactants, based on the formation of the ionic pair anionic surfactant-methylene blue (AS-MB). This method, in relation to the conventional analytic procedure, considerably reduces not only the quantity of chloroform used in extracting the ionic pair formed, but also the time and the quantity of sample necessary to perform the assay, eliminating the filtration stage. The method has been simplified by displacing the transfer equilibrium of the ionic pair AS-MB towards the organic phase, augmenting the volumetric relationship of chloroform/sample. The method proposed has been applied in the study of primary biodegradation kinetics of linear alkylbenzenesulfonate (LAS).
在本研究中，我们提出了一种基于阴离子表面活性剂-亚甲蓝离子对（AS-MB）形成来测定阴离子表面活性剂的简化分光光度法。与传统分析方法相比，该方法不仅大大减少了用于萃取所形成离子对的氯仿用量，还减少了进行分析所需的时间和样品量，省去了过滤步骤。通过将离子对AS-MB的转移平衡向有机相移动，增加氯仿/样品的体积关系，简化了该方法。所提出的方法已应用于直链烷基苯磺酸盐（LAS）的初级生物降解动力学研究。
