Ikeda Ikuo, Konno Rie, Shimizu Takeshi, Ide Takashi, Takahashi Nobuyuki, Kawada Teruo, Nagao Koji, Inoue Nao, Yanagita Teruyoshi, Hamada Tadateru, Morinaga Yae, Tomoyori Hiroko, Imaizumi Katsumi, Suzuki Kunio
Laboratory of Food and Biomolecular Science, Department of Food Function and Health, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amemiyamachi, Aoba-ku, Sendai 981-8555, Japan.
Biochim Biophys Acta. 2006 May;1760(5):800-7. doi: 10.1016/j.bbagen.2006.02.017. Epub 2006 Mar 23.
Dietary campest-5-en-3-one (campestenone), an oxidized derivative of campesterol, significantly reduced visceral fat weight and the concentration of triacylglycerol in serum and liver of rats. Dietary campestenone dramatically increased the activities and the mRNA expressions of mitochondrial and peroxisomal enzymes involved in beta-oxidation in the liver. Campestenone activated human peroxisome proliferator-activated receptor (PPAR) alpha as determined using the novel GAL4 ligand-binding domain chimera assay system with coactivator coexpression. In contrast, dietary campestenone reduced the activities and the mRNA expressions of enzymes involved in fatty acid synthesis, except for the malic enzyme. Dietary campestenone decreased the sterol regulatory element binding protein-1 (SREBP-1) mRNA level. Energy expenditure was significantly higher in the feeding of campestenone in rats. Dietary campestenone reduced hepatic cholesterol concentration and increased fecal excretion of neutral steroids originated from cholesterol. Lymphatic absorption of cholesterol was reduced by the coadministration of campestenone in rats cannulated in the thoracic duct. These observations suggest a possibility that campestenone has an ability to prevent coronary heart disease by improving obesity and abnormality of lipid metabolism.
膳食中的菜油甾-5-烯-3-酮（菜油甾烯酮）是菜油甾醇的氧化衍生物，能显著降低大鼠的内脏脂肪重量以及血清和肝脏中三酰甘油的浓度。膳食中的菜油甾烯酮显著提高了肝脏中参与β-氧化的线粒体和过氧化物酶体酶的活性及mRNA表达。使用新型GAL4配体结合域嵌合体分析系统并共表达共激活因子测定发现，菜油甾烯酮激活了人类过氧化物酶体增殖物激活受体（PPAR）α。相比之下，膳食中的菜油甾烯酮降低了参与脂肪酸合成的酶（苹果酸酶除外）的活性及mRNA表达。膳食中的菜油甾烯酮降低了固醇调节元件结合蛋白-1（SREBP-1）的mRNA水平。给大鼠喂食菜油甾烯酮时，能量消耗显著更高。膳食中的菜油甾烯酮降低了肝脏胆固醇浓度，并增加了源自胆固醇的中性类固醇的粪便排泄量。在胸导管插管的大鼠中，同时给予菜油甾烯酮可减少胆固醇的淋巴吸收。这些观察结果表明，菜油甾烯酮有可能通过改善肥胖和脂质代谢异常来预防冠心病。
