在小鼠哮喘模型中，慢性吸入抗原所诱导的耐受性并非由内毒素介导。

Tolerance induced by chronic inhaled antigen in a murine asthma model is not mediated by endotoxin.

作者信息

Schramm C M, Guernsey L, Secor E, Thrall R S

机构信息

Department of Pediatrics, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA.

出版信息

Biochim Biophys Acta. 2006 May;1762(5):499-501. doi: 10.1016/j.bbadis.2006.02.002. Epub 2006 Mar 9.

DOI:10.1016/j.bbadis.2006.02.002

PMID:16616459

Abstract

Ovalbumin (OVA)-sensitized wildtype (WT) and endotoxin-resistant (ER) mice developed similar degrees of airways eosinophilia and serum OVA-specific IgE levels after acute aerosolized OVA challenge. WT mice demonstrated methacholine hyperreactivity, whereas ER mice showed no change in responsiveness. With chronic aerosolized OVA challenge, both WT and ER mice developed local tolerance, with resolution of airway eosinophilia but persistence of anti-OVA IgE in serum. Thus, the development of local tolerance with chronic aerosol exposure to OVA is independent of any potential effects of endotoxin in the OVA aerosol solution.

摘要

卵清蛋白（OVA）致敏的野生型（WT）小鼠和内毒素抗性（ER）小鼠在急性雾化OVA激发后，气道嗜酸性粒细胞增多程度和血清OVA特异性IgE水平相似。WT小鼠表现出对乙酰甲胆碱的高反应性，而ER小鼠的反应性没有变化。在慢性雾化OVA激发后，WT和ER小鼠均产生了局部耐受，气道嗜酸性粒细胞增多症状消退，但血清中抗OVA IgE持续存在。因此，慢性雾化暴露于OVA时局部耐受的形成与OVA气溶胶溶液中内毒素的任何潜在影响无关。