Boujelben Manel, Ghorbel Fatma, Vincent Christian, Makni-Ayadi Fatma, Guermazi Fadhel, Croute Françoise, El-Feki Abelfettah
Laboratoire d'Ecophysiologie Animale de la Faculté des Sciences de Sfax, BP. 802-3018 Sfax, Tunisie.
Exp Toxicol Pathol. 2006 Jul;57(5-6):437-43. doi: 10.1016/j.etp.2006.02.012. Epub 2006 Apr 17.
to determine whether magnesium (Mg) supplementation could have a protective effect against the cadmium (Cd)-induced oxidative stress in liver, kidneys and testes of adult male rats. Stress was evaluated by measuring lipid peroxidation by thiobarbituric acid reactive substances (TBARS) and the heat shock protein (HSP) 72/73 expression. CdCl2 injections (2.5mg/day/kg body weight) for 10 days resulted in a time dependent increase of Cd accumulation in liver, kidney and testes, the highest levels being found in liver (400 microg/g dried tissue). At the same time, an increase of lipid peroxidation was observed. The effect was maximal at day 1 of Cd treatment in liver and testes, and later (day 5) in kidney. Then, Cd-induced lipid peroxidation decreased, suggesting the activation of antioxidant defense mechanisms. Injections of Mg SO4 (300-600 mg/day/kg body weight) reduced in a dose-dependent manner Cd-induced lipid peroxidation in liver and kidney as well as the accumulation of Cd in liver, kidney and testes. In testes, a protective effect of Mg was found only during the early phase of Cd-poisoning. On days 5 and 10, lipid peroxidation was even increased as compared to controls. In liver and testes only the constitutive HSP73 was detected whereas in kidney both HSP73 and the inducible HSP72 were expressed. HSP72/73 expression was not significantly increased by Cd and HSP73 was even lowered in kidney, probably due to the strong dose used. These results were not modified by Mg injections.
Mg supplementation can reduce Cd accumulation in organs and lipid peroxidation related to Cd administration.
确定补充镁（Mg）是否能对成年雄性大鼠肝脏、肾脏和睾丸中镉（Cd）诱导的氧化应激产生保护作用。通过硫代巴比妥酸反应性物质（TBARS）测量脂质过氧化以及热休克蛋白（HSP）72/73表达来评估应激。连续10天注射氯化镉（2.5mg/天/千克体重）导致肝脏、肾脏和睾丸中镉积累呈时间依赖性增加，肝脏中积累水平最高（400微克/克干组织）。同时，观察到脂质过氧化增加。在肝脏和睾丸中，镉处理第1天效应最大，在肾脏中则在第5天最大。然后，镉诱导的脂质过氧化减少，表明抗氧化防御机制被激活。注射硫酸镁（300 - 600mg/天/千克体重）以剂量依赖性方式降低了肝脏和肾脏中镉诱导的脂质过氧化以及肝脏、肾脏和睾丸中镉的积累。在睾丸中，仅在镉中毒早期发现镁有保护作用。在第5天和第10天，与对照组相比脂质过氧化甚至增加。在肝脏和睾丸中仅检测到组成型HSP73，而在肾脏中HSP73和诱导型HSP72均有表达。镉未显著增加HSP72/73表达，肾脏中HSP73甚至降低，可能是由于使用的剂量较大。这些结果未因注射镁而改变。
补充镁可减少器官中镉的积累以及与镉给药相关的脂质过氧化。
