Bulat Zorica, Dukić-Ćosić Danijela, Antonijević Biljana, Bulat Petar, Vujanović Dragana, Buha Aleksandra, Matović Vesna
Department of Toxicology "Akademik Danilo Soldatović", Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia.
ScientificWorldJournal. 2012;2012:572514. doi: 10.1100/2012/572514. Epub 2012 Jun 4.
The present study is designed to investigate whether magnesium (Mg) supplementation may prevent Cd-induced alterations in zinc (Zn), copper (Cu), and magnesium (Mg) status in rabbits. For this purpose, the concentrations of Zn, Cu, and Mg were estimated in blood, urine, and organs (brain, heart, lungs, liver, kidney, spleen, pancreas, skeletal muscle, and bone) of rabbits given Cd (10 mg/kg b.w.) and rabbits cotreated with Mg (40 mg/kg b.w.) orally, as aqueous solutions of Cd chloride and Mg acetate every day for 4 weeks. Samples were mineralized with conc. HNO₃ and HClO₄ (4:1) and metals concentrations were determined by atomic absorption spectrophotometry (AAS). Magnesium supplementation succeeded to overcome Cd-induced disbalance of investigated bioelements. Beneficial effects of Mg were observed on Zn levels in blood and urine, on Cu levels in urine, and on Mg levels in blood. Magnesium pretreatment also managed to counteract or reduce all Cd-induced changes in levels of Cu and Mg in organs, while it did not exert this effect on Zn levels. These findings suggest that enhanced dietary Mg intake during Cd exposure can have at least partly beneficial effect on Cd-induced alterations in homeostasis of zinc, copper, and magnesium.
本研究旨在调查补充镁（Mg）是否可以预防镉（Cd）诱导的家兔锌（Zn）、铜（Cu）和镁（Mg）状态的改变。为此，对口服给予氯化镉水溶液和醋酸镁水溶液（每天一次，持续4周，Cd剂量为10 mg/kg体重，Mg剂量为40 mg/kg体重）的家兔的血液、尿液和器官（脑、心脏、肺、肝脏、肾脏、脾脏、胰腺、骨骼肌和骨骼）中的Zn、Cu和Mg浓度进行了估算。样品用浓硝酸和高氯酸（4:1）进行矿化处理，金属浓度通过原子吸收分光光度法（AAS）测定。补充镁成功克服了镉诱导的所研究生物元素的失衡。观察到镁对血液和尿液中的锌水平、尿液中的铜水平以及血液中的镁水平具有有益作用。镁预处理还成功抵消或减少了镉诱导的器官中铜和镁水平的所有变化，而对锌水平没有这种作用。这些发现表明，在镉暴露期间增加膳食镁摄入量对镉诱导的锌、铜和镁稳态改变至少可以产生部分有益影响。
