视网膜中泛连接蛋白家族蛋白质的表达

Expression of pannexin family of proteins in the retina.

作者信息

Dvoriantchikova Galina, Ivanov Dmitry, Panchin Yuri, Shestopalov Valery I

机构信息

Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine,Miami, FL 33136, USA.

出版信息

FEBS Lett. 2006 Apr 17;580(9):2178-82. doi: 10.1016/j.febslet.2006.03.026.

DOI:10.1016/j.febslet.2006.03.026

PMID:16616526

Abstract

Expression of the Panx1 and Panx2 members of the pannexin family of gap junction proteins was studied in the retina by in situ hybridization and qRT-PCR. Both pannexins showed robust expression across the retina with predominant accumulation in the retinal ganglion cells (RGCs). In concordance, immunohistochemical analysis showed accumulation of the Panx1 protein in RGCs, amacrine, horizontal cells and their processes. Two Panx1 isoforms were detected: a ubiquitously expressed 58 kDa protein, and a 43 kDa isoform that specifically accumulated in the retina and brain. Our results indicated that Panx1 and Panx2 are abundantly expressed in the retina, and may therefore contribute to the electrical and metabolic coupling, or to signaling between retinal neurons via the secondary messengers.

摘要

通过原位杂交和定量逆转录聚合酶链反应（qRT-PCR），研究了视网膜中缝隙连接蛋白泛连接蛋白家族的Panx1和Panx2成员的表达情况。两种泛连接蛋白在整个视网膜中均有强烈表达，主要聚集在视网膜神经节细胞（RGC）中。与此一致，免疫组织化学分析显示Panx1蛋白在RGC、无长突细胞、水平细胞及其突起中聚集。检测到两种Panx1异构体：一种普遍表达的58 kDa蛋白，以及一种特异性聚集在视网膜和大脑中的43 kDa异构体。我们的结果表明，Panx1和Panx2在视网膜中大量表达，因此可能有助于视网膜神经元之间通过第二信使进行电耦合和代谢耦合或信号传递。