Valcarce C, Cuezva J M
Departamento de Biología Molecular, Universidad Autónoma de Madrid, Spain.
FEBS Lett. 1991 Dec 9;294(3):225-8. doi: 10.1016/0014-5793(91)81435-b.
2-h-old neonatal liver mitochondria, when depleted of adenine nucleotides, showed an 'ohmic' current-voltage relationship and a higher passive proton permeability of the membrane, resembling fetal mitochondrial behaviors for the proton conductance. Incubation of fetal mitochondria with ATP, GDP or carboxyatractyloside promoted a significant reduction in the passive proton permeability of the membrane and the appearance of the characteristic biphasic behavior for the proton conductance. It is concluded that the postnatal increase in intramitochondrial adenine nucleotide concentration promotes, by the interaction of the nucleotides with the adenine nucleotide translocase, the reduction in the passive proton permeability of the mitochondrial membrane, allowing efficient energy conservation in the neonatal liver.
出生2小时的新生鼠肝脏线粒体，当腺嘌呤核苷酸耗尽时，呈现“欧姆”电流-电压关系，且膜的被动质子通透性较高，类似于胎儿线粒体在质子传导方面的行为。用ATP、GDP或羧基苍术苷孵育胎儿线粒体，可显著降低膜的被动质子通透性，并出现质子传导的特征性双相行为。结论是，线粒体内腺嘌呤核苷酸浓度在出生后的增加，通过核苷酸与腺嘌呤核苷酸转位酶的相互作用，促进线粒体膜被动质子通透性的降低，从而使新生鼠肝脏能够有效地保存能量。
