Mizuno Noriyoshi, Shiba Hideki, Ozeki Yoshitaka, Mouri Yoshihiro, Niitani Miyuki, Inui Takafumi, Hayashi Hideaki, Suzuki Koji, Tanaka Seishin, Kawaguchi Hiroyuki, Kurihara Hidemi
Department of Periodontal Medicine, Division of Frontier Medical Science, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Cell Biol Int. 2006 Jun;30(6):521-4. doi: 10.1016/j.cellbi.2006.01.010. Epub 2006 Mar 6.
The major problem in cell therapy is the possibility of viral or bacterial infection and immune reactions. Therefore, it is expected of culture cells which are intended to be re-implanted with autologous serum rather than conventional bovine serum. Cell therapy with human mesenchymal stem cells (hMSC), differentiating to various cells, is thought to be curative. To culture hMSC with human autologous serum (HAS) and re-implant them for cell therapy, we developed a completely closed bag system separating serum, comparing proliferation and multipotency of hMSC cultured in HAS with those in foetal calf serum (FCS). HAS was simply, safely and efficiently obtained with the developed closed bag system. Cell proliferation of hMSC cultured in HAS was greater than that in FCS. hMSC, exposed to the defined induction medium containing HAS as well as FCS, differentiated into osteoblasts and adipocytes. These findings suggest that HAS obtained with the developed closed bag system is advantageous in a point of decrease in risk of virus or bacterial infection and foreign protein contamination and enhancement of proliferation of hMSC.
细胞治疗中的主要问题是存在病毒或细菌感染以及免疫反应的可能性。因此，人们期望用于重新植入的培养细胞使用自体血清而非传统的牛血清。用人类间充质干细胞（hMSC）进行细胞治疗，hMSC可分化为各种细胞，被认为具有治疗作用。为了使用人类自体血清（HAS）培养hMSC并将其重新植入用于细胞治疗，我们开发了一种完全封闭的袋式系统来分离血清，比较在HAS中培养的hMSC与在胎牛血清（FCS）中培养的hMSC的增殖能力和多能性。通过开发的封闭袋式系统可以简单、安全且高效地获取HAS。在HAS中培养的hMSC的细胞增殖能力大于在FCS中培养的hMSC。暴露于含有HAS以及FCS的特定诱导培养基中的hMSC可分化为成骨细胞和脂肪细胞。这些发现表明，通过开发的封闭袋式系统获取的HAS在降低病毒或细菌感染风险以及外来蛋白质污染风险和增强hMSC增殖方面具有优势。
