Chen Feng-ying, Guo Yan-hong, Gao Wei, Feng Xin-heng, Chen Li, Tang Chao-shu
Department of Cardiology, Peking University Third Hospital, Beijing 100083, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Apr 18;38(2):179-83.
To study the effects of hyperhomocysteinemia on cardiac remodeling and systolic function.
Twenty-four Wistar rats were divided randomly into experiment and control group. 12 rats of experiment group were bred with 1 g/(kg.d) L-methionine. After 4 weeks, 6 of them were killed (HHcy), others were continuously bred with normal diet for 4 weeks (Quit hcy, QHcy). Twelve rats of control group with normal diet for 4 and 8 weeks. The plasma Hcy concentrations were measured by immuno-fluorescence technique. Left ventricular structure and systolic function were assessed by echocardiography. The cardiac morphology and collagen were studied by optical microscopy and image analysis system after hematoxylin eosin-staining and picrosirius red-staining. Immuno-histochemistry for actin-smooth muscle and factor VIII-related antigen was performed to identify changes in cardiac smaller arteriolar and microvascular numbers.
The plasma Hcy concentration was (106.19+/-19.75) micromol/L in HHcy, (6.52+/-0.94) micromol/L in QHcy and (4.90+/-0.10) micromol/L in the control. Left ventricular systolic function was decreased 10.7% in HHcy. Left ventricular wall strain and cardiac smaller arteriolar wall thickness were increased by 62.1% and 2.9 fold in HHcy, 27.1% and 2.4 fold in QHcy as compared with the control. Cardiomyocyte diameter was not changed in HHcy and increased by 15.4% in QHcy as compared with the control. Microvascular number was decreased by 45.9% in HHcy as compared with the control, but smaller arteriolar number did not differ among the groups. Left ventricular systolic function and microvascular number resumed in QHcy. Interstitial and perivascular collagen deposition was significantly increased in HHcy and QHcy.
Hyperhomocysteinemia may increase cardiomyocyte diameter, cardiac smaller arteriolar wall thickness and left ventricular wall strain; decrease microvascular number; induce interstitial and perivascular collagen deposition, directly and indirectly affect on cardiac remodeling and systolic function. The cardiac remodeling couldn't resume completely with plasma Hcy concentration being decreased.
研究高同型半胱氨酸血症对心脏重塑及收缩功能的影响。
将24只Wistar大鼠随机分为实验组和对照组。实验组12只大鼠以1 g/(kg·d)的L-蛋氨酸进行饲养。4周后，其中6只被处死（HHcy组），其余大鼠继续以正常饮食饲养4周（戒同型半胱氨酸组，QHcy组）。对照组12只大鼠以正常饮食饲养4周和8周。采用免疫荧光技术测定血浆同型半胱氨酸（Hcy）浓度。通过超声心动图评估左心室结构和收缩功能。苏木精伊红染色和苦味酸天狼星红染色后，利用光学显微镜和图像分析系统研究心脏形态和胶原。进行肌动蛋白-平滑肌和因子VIII相关抗原的免疫组织化学检测，以确定心脏小动脉和微血管数量的变化。
HHcy组血浆Hcy浓度为(106.19±19.75) μmol/L，QHcy组为(6.52±0.94) μmol/L，对照组为(4.90±0.10) μmol/L。HHcy组左心室收缩功能下降了10.7%。与对照组相比，HHcy组左心室壁应变和心脏小动脉壁厚度分别增加了62.1%和2.9倍，QHcy组分别增加了27.1%和2.4倍。HHcy组心肌细胞直径未改变，QHcy组较对照组增加了15.4%。与对照组相比，HHcy组微血管数量减少了45.9%，但各组小动脉数量无差异。QHcy组左心室收缩功能和微血管数量恢复。HHcy组和QHcy组间质和血管周围胶原沉积显著增加。
高同型半胱氨酸血症可能增加心肌细胞直径、心脏小动脉壁厚度和左心室壁应变；减少微血管数量；诱导间质和血管周围胶原沉积，直接和间接影响心脏重塑和收缩功能。血浆Hcy浓度降低后，心脏重塑不能完全恢复。
