[An assessment of peripheral blood lymphocytes populations and subpopulation in patients with stomach cancer].

AIM

In the present study, we conducted the quantitative analysis of peripheral blood lymphocytes population and subpopulation in patients with stomach cancer, based on flow cytometry immunophenotyping.

MATERIAL AND METHOD

The results were compared with the advancement of the disease (UICC/TNM), histological type (Lauren classification), and grade of differentiation of stomach cancer. Lymphocyte CD: (CD3/CD19, CD3/CD4, CD3/CD8, CD3/CD16, 56) were determined in 50 cancer patients and 30 healthy humans.

RESULTS

The significant deficiency of total lymphocyte number, T and B lymphocytes, as well as CD4+ and CD8+ was found in stomach cancer patients. There was no depletion in NK cells number. In advanced stomach cancer, the number of NK cells decreased, along with further deterioration of lymphocytes T and B populations. In patients with diffuse-type of stomach cancer and III or IV stage, the more pronounced deficiency in T, CD4+ and CD8+ population was noted. The decrease grade of differentiation was correlated with the increase of NK cell population. There was no correlation between the number of B lymphocytes and the histologic type or grade of differentiation of stomach cancer.

CONCLUSION

The obtained data demonstrated significant quantitative defect in defence of stomach cancer patients.