Osada Joanna, Kamocki Zbigniew, Pietruczuk Miroslawa, Dabrowska Milena, Kedra Bogusław
Akademia Medyczna w Białymstoku, Zakład Diagnostyki Hematologicznej.
Pol Merkur Lekarski. 2006 Jan;20(115):26-31.
In the present study, we conducted the quantitative analysis of peripheral blood lymphocytes population and subpopulation in patients with stomach cancer, based on flow cytometry immunophenotyping.
The results were compared with the advancement of the disease (UICC/TNM), histological type (Lauren classification), and grade of differentiation of stomach cancer. Lymphocyte CD: (CD3/CD19, CD3/CD4, CD3/CD8, CD3/CD16, 56) were determined in 50 cancer patients and 30 healthy humans.
The significant deficiency of total lymphocyte number, T and B lymphocytes, as well as CD4+ and CD8+ was found in stomach cancer patients. There was no depletion in NK cells number. In advanced stomach cancer, the number of NK cells decreased, along with further deterioration of lymphocytes T and B populations. In patients with diffuse-type of stomach cancer and III or IV stage, the more pronounced deficiency in T, CD4+ and CD8+ population was noted. The decrease grade of differentiation was correlated with the increase of NK cell population. There was no correlation between the number of B lymphocytes and the histologic type or grade of differentiation of stomach cancer.
The obtained data demonstrated significant quantitative defect in defence of stomach cancer patients.
在本研究中，我们基于流式细胞术免疫表型分析对胃癌患者外周血淋巴细胞群体和亚群进行了定量分析。
将结果与疾病进展（UICC/TNM）、组织学类型（劳伦分类）和胃癌分化程度进行比较。测定了50例癌症患者和30例健康人的淋巴细胞CD：（CD3/CD19、CD3/CD4、CD3/CD8、CD3/CD16、56）。
胃癌患者的总淋巴细胞数、T淋巴细胞和B淋巴细胞以及CD4+和CD8+明显缺乏。NK细胞数量没有减少。在进展期胃癌中，NK细胞数量减少，同时淋巴细胞T和B群体进一步恶化。在弥漫型胃癌且处于III或IV期的患者中，T、CD4+和CD8+群体的缺乏更为明显。分化程度降低与NK细胞群体增加相关。B淋巴细胞数量与胃癌的组织学类型或分化程度之间没有相关性。
获得的数据表明胃癌患者的防御存在明显的定量缺陷。
