Tsukamoto Yasuhiro, Tsuchiya A Seiichiro, Namikawa Takashi, Tatesaki Ryouta, Adachi Kazuhide, Hiroi Satoshi, Furuya Masaru, Sari Dwi Kesuma, Kotani Takao
Laboratory of Veterinary Anatomy and Pathology, Graduate School of Biology and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Sakai, Osaka 599-8531, Japan.
Avian Dis. 2006 Mar;50(1):127-30. doi: 10.1637/7407-071305R1.1.
Calbindin-D28K (Ca-D28K) is a calcium-binding protein. In the kidney, Ca-D28K is present in the distal nephron, but not in the proximal nephron. This site-specific distribution in the kidney indicates that Ca-D28K is a potential marker for the differentiation of the distal nephron. In this study, we have examined the expression of Ca-D28K in 25 sporadic cases of chicken nephroblastomas. All cases of nephroblastomas were composed of atypical tubular structures, blastemal cells, and fibrous stroma in varying degrees of differentiation. Immunohistochemically in all nephroblastoma specimens, Ca-D28K was expressed in the epithelial cells of the subsets of tubular structures, but not in the blastema or the stroma. These results suggested that the tubuli in the nephroblastomas are able to differentiate into the phenotype of distal nephrons. Furthermore, Ca-D28K might develop as a novel diagnostic marker for nephroblastomas because this molecule is reported to be completely negative in other renal tumors, including renal cell carcinoma, chromophobe carcinomas, and oncocytoma.
钙结合蛋白-D28K（Ca-D28K）是一种钙结合蛋白。在肾脏中，Ca-D28K存在于远端肾单位，而近端肾单位中不存在。在肾脏中的这种位点特异性分布表明Ca-D28K是远端肾单位分化的潜在标志物。在本研究中，我们检测了25例散发性鸡肾母细胞瘤中Ca-D28K的表达。所有肾母细胞瘤病例均由不同程度分化的非典型管状结构、胚基细胞和纤维性间质组成。在所有肾母细胞瘤标本中，免疫组织化学显示Ca-D28K在管状结构亚群的上皮细胞中表达，但在胚基或间质中不表达。这些结果表明肾母细胞瘤中的小管能够分化为远端肾单位的表型。此外，Ca-D28K可能会成为一种新的肾母细胞瘤诊断标志物，因为据报道该分子在其他肾肿瘤中完全呈阴性，包括肾细胞癌、嫌色细胞癌和嗜酸细胞瘤。
