Sobel Richard E, Wang Yuzhuo, Sadar Marianne D
Michael Smith Genome Sciences, Vancouver, British Columbia, Canada.
In Vitro Cell Dev Biol Anim. 2006 Jan-Feb;42(1-2):33-9. doi: 10.1007/s11626-006-0009-7.
Adenocarcinoma of the prostate comprises 95% of all prostate cancer. Commercially available primary cultures of "normal" prostate epithelial cells, PrECs, have been used as a convenient model to investigate neoplastic transformation. Here PrECs were characterized for the expression of lineage- and developmental-specific markers cytokeratin (CK) 8 and 18, p63, chromogranin A, TMEPAI, S100P, NKX 3.1, ANKH, and FN 1 as well as androgen receptor and prostate-specific antigen by Western blot and Northern blot analyses, immunohistochemistry, reverse transcriptase-polymerase chain reaction (RT-PCR), and quantitative real-time PCR. Immunohistochemical staining detected PrECs positive in varying degrees for p63, CK 8, and CK 18, with only the rare cell being positive for chromogranin A. The PrECs also tested positive for p63 protein by Western blot analysis. RT-PCR with PrEC cDNA showed products for FN 1 and S100P but not for ANKH and androgen receptor or prostate-specific antigen. This profile of markers in PrEC cells is consistent with that expected for pubertal prostate epithelial cells.
前列腺腺癌占所有前列腺癌的95%。市售的“正常”前列腺上皮细胞原代培养物（PrECs）已被用作研究肿瘤转化的便捷模型。在此，通过蛋白质免疫印迹和Northern印迹分析、免疫组织化学、逆转录聚合酶链反应（RT-PCR）和定量实时PCR，对PrECs进行了谱系和发育特异性标志物细胞角蛋白（CK）8和18、p63、嗜铬粒蛋白A、TMEPAI、S100P、NKX 3.1、ANKH和FN 1以及雄激素受体和前列腺特异性抗原表达的特征分析。免疫组织化学染色检测到PrECs对p63、CK 8和CK 18呈不同程度阳性，仅极少数细胞对嗜铬粒蛋白A呈阳性。蛋白质免疫印迹分析显示PrECs对p63蛋白也呈阳性。用PrEC cDNA进行RT-PCR显示有FN 1和S100P的产物，但没有ANKH、雄激素受体或前列腺特异性抗原的产物。PrEC细胞中的这种标志物谱与青春期前列腺上皮细胞预期的谱一致。
