一种新型的N-氯代牛磺酸-皮质类固醇组合作为无防腐剂的局部消毒剂：对体外睫状肌跳动频率的影响

A novel N-chlorotaurine-corticosteroid combination as a preservative-free local disinfectant: influence on the ciliary beat frequency in vitro.

作者信息

Arnitz Roland, Ott Helmut Werner, Gstöttner Michaela, Nagl Markus, Scholtz Arne W, Neher Andreas

机构信息

Department of Otorhinolaryngology, Innsbruck Medical University, Innsbruck, Austria.

出版信息

Acta Otolaryngol. 2006 Mar;126(3):291-4. doi: 10.1080/00016480500388935.

DOI:10.1080/00016480500388935

PMID:16618657

Abstract

CONCLUSIONS

The combination of N-chlorotaurine (NCT) and a corticosteroid seems to be a very promising substance for the local therapy of ENT infections. As it can be used without any preservatives, the effect on the ciliary beat frequency (CBF) is much less than that of products containing benzalkonium chloride (BAC). The in vitro results obtained in this study encourage us to perform clinical trials on this novel combination for intranasal application.

OBJECTIVE

To investigate the influence of a novel mixture of NCT and a corticosteroid [fluticasone propionate (FP)] on the CBF of human ciliated cells in vitro.

MATERIAL AND METHODS

The study was designed as an in vitro study. CBF was measured by means of a photometric technique involving the combination of a light microscope, a photometer, a photographic multiplier and a computerized analyzing unit.

RESULTS

The combination of 1% NCT + 0.5 mg/ml FP decreased the CBF to 42.17% of its original value after 20 min. Treatment with BAC lowered the CBF depending on the concentration to 96.61% of its original value with 0.04 mg/ml, to 91.90% with 0.1 mg/ml, to 63.46% with 0.2 mg/ml and to 0% with 0.5 mg/ml. After rinsing in saline, the CBF of samples treated with 1% NCT + 0.5 mg/ml FP recovered to 68.93% of its original value.

摘要

结论

N-氯代牛磺酸（NCT）与皮质类固醇的组合似乎是用于耳鼻喉感染局部治疗的非常有前景的物质。由于其可在无任何防腐剂的情况下使用，对纤毛摆动频率（CBF）的影响远小于含苯扎氯铵（BAC）的产品。本研究获得的体外结果鼓励我们对这种新型组合进行鼻内应用的临床试验。

目的

研究NCT与皮质类固醇[丙酸氟替卡松（FP）]的新型混合物对人纤毛细胞体外CBF的影响。

材料与方法

本研究设计为体外研究。CBF通过光度技术测量，该技术涉及光学显微镜、光度计、光电倍增管和计算机分析单元的组合。

结果

1% NCT + 0.5 mg/ml FP的组合在20分钟后使CBF降至其原始值的42.17%。用BAC处理会使CBF根据浓度降低，0.04 mg/ml时降至其原始值的96.61%，0.1 mg/ml时降至91.9%，0.2 mg/ml时降至63.46%，0.5 mg/ml时降至0%。用生理盐水冲洗后，用1% NCT + 0.5 mg/ml FP处理的样品的CBF恢复至其原始值的68.93%。