促肾上腺皮质激素（ACTH）与促肾上腺皮质激素释放激素对大鼠肾上腺体外类固醇生成作用的比较。

Comparison of ACTH and corticotropin-releasing hormone effects on rat adrenal steroidogenesis in vitro.

作者信息

Neri G, Andreis P G, Nussdorfer G G

机构信息

Department of Anatomy, University of Padua, Italy.

出版信息

Res Exp Med (Berl). 1991;191(5):291-5. doi: 10.1007/BF02576685.

DOI:10.1007/BF02576685

PMID:1661910

Abstract

The effects of equimolar concentrations (10(-9) M) of ACTH and corticotropin-releasing hormone (CRH) on the secretory activity of zona glomerulosa (ZG) and zonae fasciculata and reticularis (ZF/ZR) of rat adrenals were investigated in vitro by high-pressure liquid chromatography. ACTH enhanced the output of all the post-progesterone steroids (11-deoxycorticosterone, 18-hydroxy-11-deoxycorticosterone, corticosterone, 18-hydroxycorticosterone, and aldosterone) both by isolated ZG or ZF/ZR cells and by adrenal slices. CRH raised the secretory activity exclusively of adrenal slices, and its effect was less than half that of ACTH. However, the extents to which the various post-progesterone hormones contributed to the ACTH- or CRH-induced rises in the overall adrenal secretory activity were similar. The hypothesis is discussed that CRH acts on the rat adrenal gland by eliciting a local production of ACTH.

摘要

通过高压液相色谱法在体外研究了等摩尔浓度（10⁻⁹M）的促肾上腺皮质激素（ACTH）和促肾上腺皮质激素释放激素（CRH）对大鼠肾上腺球状带（ZG）以及束状带和网状带（ZF/ZR）分泌活性的影响。ACTH可增强分离的ZG或ZF/ZR细胞以及肾上腺切片中所有孕烯醇酮后甾体（11-脱氧皮质酮、18-羟基-11-脱氧皮质酮、皮质酮、18-羟基皮质酮和醛固酮）的分泌量。CRH仅提高肾上腺切片的分泌活性，其作用不到ACTH的一半。然而，各种孕烯醇酮后激素对ACTH或CRH诱导的肾上腺总体分泌活性升高的贡献程度相似。文中讨论了CRH通过引发ACTH的局部产生而作用于大鼠肾上腺的假说。