Orlova Anna, Bruskin Alexander, Sivaev Igor, Sjöberg Stefan, Lundqvist Hans, Tolmachev Vladimir
Unit of Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, S-751 85 Uppsala, Sweden.
Anticancer Res. 2006 Mar-Apr;26(2A):1217-23.
Negatively-charged polyhedral boron clusters can be easily halogenated with highly stable boron-halogen bonds and are promising in radionuclide diagnostics and cancer therapy.
The radio-iodination conditions for the closo-dodecaborate anion and for the conjugation of its labeled isothiocyanatobenzylammonio derivative to the monoclonal antibody (mAb) were optimized.
The labeling yield was about 90% and the overall conjugation yield was 55-60%. The in vitro stability of the radio-iodinated mAb was good under physiological and non-physiological conditions. The immunoreactivity of the labeled mAb (SK-BR-3 cells) was retained in the one-pot two-step labeling.
Negatively-charged polyhedral boron clusters can be used for indirect radio-iodination of mAbs.
