Zhu Xuhui, Mao Zebin, Na Yanqun, Guo Yinglu, Wang Xianghong, Xin Dianqi
Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, Beijing 100034, China.
Anticancer Res. 2006 Mar-Apr;26(2A):1253-9.
Recently, the pituitary tumor transforming gene 1 (PTTG1) has been suggested to be an oncogene. To investigate whether PTTG1 plays a positive role in the pathogenesis of prostate cancer, PTTG1 protein expression was examined in prostate tissue samples by immunohistochemistry. PTTG1 expression was detected in a high percentage of prostate cancer tissues (34/41, 82.9%), but to a much lesser extent in non-malignant tissues (5/14, 35.7%). To further confirm these results, the expression vectors containing either the PTTG1 or antisense-PTTG1 gene were transfected into a prostate cancer cell line, LNCaP, and the cell proliferation rate was studied, as well as tumorigenicity in the LNCaP cells expressing different levels of the PTTG1 protein. Ectopic PTTG1 gene expression promoted prostate cancer cell proliferation and tumorigenesis both in vitro and in nude mice. In contrast, down-regulation of PTTG1 led to suppression of tumor cell growth. These results suggest that PTTG1 may be a potential prognostic marker for prostate cancer and that the down-regulation of PTTG1 may be a therapeutic target in the suppression of prostate cancer growth.
最近，垂体瘤转化基因1（PTTG1）被认为是一种癌基因。为了研究PTTG1在前列腺癌发病机制中是否起积极作用，通过免疫组织化学检测了前列腺组织样本中PTTG1蛋白的表达。在高比例的前列腺癌组织（34/41，82.9%）中检测到PTTG1表达，但在非恶性组织中表达程度要低得多（5/14，35.7%）。为了进一步证实这些结果，将含有PTTG1或反义PTTG1基因的表达载体转染到前列腺癌细胞系LNCaP中，研究细胞增殖率以及在表达不同水平PTTG1蛋白的LNCaP细胞中的致瘤性。异位PTTG1基因表达在体外和裸鼠体内均促进前列腺癌细胞增殖和肿瘤发生。相反，PTTG1的下调导致肿瘤细胞生长受到抑制。这些结果表明，PTTG1可能是前列腺癌的一个潜在预后标志物，并且PTTG1的下调可能是抑制前列腺癌生长的一个治疗靶点。
