Apoptosis in cervical cancer after balloon-occluded arterial infusion of anticancer drugs.
作者信息
Kokawa Katsuji, Mabuchi Yasushi, Tanaka Kazuharu, Yagi Shigetaka, Utsunomiya Tomoko, Oba Nami, Yata Chie, Umesaki Naohiko
机构信息
Department of Obstetrics and Gynecology, Wakayama Medical University, Wakayama 641-0012, Japan.
出版信息
Anticancer Res. 2006 Mar-Apr;26(2B):1413-8.
BACKGROUND
This study was designed to investigate the relationship between apoptosis and Bcl-2 and Bax expressions in uterine cervical cancer after balloon-occluded arterial infusion (BOAI).
MATERIALS AND METHODS
Twenty-four specimens were obtained before and after BOAI. The occurrence of apoptosis was examined with molecular biochemical techniques. The expressions of Bcl-2 and Bax proteins were investigated by immunohistochemical staining.
RESULTS
Labelling of DNA in situ indicated that apoptotic cells were sporadically seen before BOAI (6.1 +/- 1.9). Apoptotic cells apparently increased at 5 days (25.1 +/- 6.4) after BOAI The autoradiographic analysis revealed that the DNA-ladder was identified at 5 days after BOAI. Although Bcl-2 immuno-reactivity was faintly detected, the expression of Bax increased at 3 days (49.4 +/- 10.4%) after BOAI.
CONCLUSION
The results indicated that treatment with BOAI resulted in transient increases of apoptosis in cervical cancer in association with the increased expression of Bax.
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