Kokawa Katsuji, Mabuchi Yasushi, Tanaka Kazuharu, Yagi Shigetaka, Utsunomiya Tomoko, Oba Nami, Yata Chie, Umesaki Naohiko
Department of Obstetrics and Gynecology, Wakayama Medical University, Wakayama 641-0012, Japan.
Anticancer Res. 2006 Mar-Apr;26(2B):1413-8.
This study was designed to investigate the relationship between apoptosis and Bcl-2 and Bax expressions in uterine cervical cancer after balloon-occluded arterial infusion (BOAI).
Twenty-four specimens were obtained before and after BOAI. The occurrence of apoptosis was examined with molecular biochemical techniques. The expressions of Bcl-2 and Bax proteins were investigated by immunohistochemical staining.
Labelling of DNA in situ indicated that apoptotic cells were sporadically seen before BOAI (6.1 +/- 1.9). Apoptotic cells apparently increased at 5 days (25.1 +/- 6.4) after BOAI The autoradiographic analysis revealed that the DNA-ladder was identified at 5 days after BOAI. Although Bcl-2 immuno-reactivity was faintly detected, the expression of Bax increased at 3 days (49.4 +/- 10.4%) after BOAI.
The results indicated that treatment with BOAI resulted in transient increases of apoptosis in cervical cancer in association with the increased expression of Bax.
