Pazarbasi Ayfer, Alptekin Davut, Luleyap H Umit, Kasap Mulkiye, Kasap Halil
Department of Medical Biology and Genetics, School of Medicine, University of Cukurova, 01330 Balcali, Adana, Turkey.
J Med Entomol. 2006 Mar;43(2):248-51. doi: 10.1603/0022-2585(2006)043[0248:uoeiaf]2.0.co;2.
In total, 320 phelebotomine sand flies from Alibozlu (Osmaniye), Kizyusuflu (Osmaniye), and Sanliurfa in southeastern Turkey were tested for the detection and identification of Leishmania in vector sand flies by enzyme-linked immunosorbent assay with species-specific monoclonal antibodies. We used monoclonal antibodies that recognize both Leishmania tropica and Leishmania major, and a monoclonal antibody specific only to L. tropica. Phosphate-buffered saline and monoclonal antibody M2 recognizing Leishmania amazonensis were used as controls. Infection rates of sand flies were 0.9% in Alibozlu, 0% Kizyusuflu, and 3.6% in Sanliurfa. Positive sand flies were identified as Phlebotomus sergenti Parrot and Phlebotomus major syriacus Adler & Theodor.
