Beerntsen Brenda T, Li Jianyong
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA.
J Med Entomol. 2006 Mar;43(2):318-22. doi: 10.1603/0022-2585(2006)043[0318:pdiwka]2.0.co;2.
Xanthurenic acid (XA) has been implicated as an inducer in vivo of exflagellation in Plasmodium spp. Consequently, the development of Plasmodium gallinaceum was assessed in a white-eye mosquito strain, kh(w), of Aedes aegypti (L.), which is deficient in XA because of a mutation of the gene encoding the enzyme kynurenine hydroxylase, and in a transformed line of kh(w) mosquitoes that carry the wild-type cn+ gene of Drosophila melanogaster Meigen and express a functional enzyme necessary for XA production. Although XA was not detectable in kh(w) mosquitoes by using high-pressure liquid chromatography with electrochemical detection, parasites were able to develop. Transformed kh(w) mosquitoes failed to consistently support parasite development at higher prevalences and mean intensities than did the nontransformed kh(w) lines, even though XA was detectable. These data suggest that factors other than XA may play a role in initiating Plasmodium development in vivo.
黄尿酸（XA）被认为是疟原虫体内配子体出丝的诱导剂。因此，在埃及伊蚊白眼品系kh(w)中评估了鸡疟原虫的发育情况，该品系由于编码犬尿氨酸羟化酶的基因突变而缺乏XA，并且在携带黑腹果蝇野生型cn+基因并表达XA产生所需功能酶的kh(w)蚊子转化系中进行了评估。尽管使用高压液相色谱-电化学检测法在kh(w)蚊子中未检测到XA，但寄生虫仍能够发育。尽管可检测到XA，但转化后的kh(w)蚊子在较高的流行率和平均强度下未能始终如一地支持寄生虫发育，与未转化的kh(w)品系相比情况不同。这些数据表明，除XA之外的其他因素可能在体内启动疟原虫发育中起作用。
