哇巴因敏感的、钾依赖性对硝基苯磷酸酶在豚鼠内耳中的细胞化学定位。基于铈的方法与基于铅的方法的评估。
Cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase in the guinea pig inner ear. Evaluation of the cerium-based method vs. the lead-based method.
作者信息
Albers F W, Van Benthem P P, De Groot J C
机构信息
Department of Otorhinolaryngology, University Hospital Utrecht, The Netherlands.
出版信息
Acta Otolaryngol. 1991;111(5):885-90. doi: 10.3109/00016489109138426.
The cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase in the guinea pig inner ear was studied using both the lead-based method and the cerium-based method. The influence of primary fixation and incubation time was investigated under both incubation conditions. A high p-NPPase activity was observed in the basolateral infoldings of the stria vascularis in the cochlea and the dark cells in the vestibular labyrinth. The stromal cells of the spiral prominence only showed a weak reaction with the lead-based method. No specific enzyme activity was found in the endolymphatic sac. The results of the present study are discussed with emphasis on the cation transport mechanisms of the inner ear.
采用铅法和铈法研究了哇巴因敏感的钾依赖性对硝基苯磷酸酶在豚鼠内耳中的细胞化学定位。在两种孵育条件下，研究了初次固定和孵育时间的影响。在耳蜗血管纹的基底外侧褶皱和前庭迷路的暗细胞中观察到高对硝基苯磷酸酶活性。螺旋隆起的基质细胞仅用铅法显示出微弱反应。在内淋巴囊中未发现特异性酶活性。本研究结果着重于内耳的阳离子转运机制进行了讨论。
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