Yoshihara T, Usami S, Igarashi M, Fermin C D
Acta Otolaryngol. 1987 Mar-Apr;103(3-4):161-9.
The localization of ouabain-sensitive, K+-dependent p-nitrophenylphosphatase (K+-NPPase) activity of the Na+, K+-ATPase complex was studied ultracytochemically in the squirrel monkey inner ear. In the stria vascularis the reaction products showing K+-NPPase activity were limited to the cytoplasmic side of the plasmalemmal infoldings of the marginal cells. In the spiral prominence, a weak reaction was also found on the cytoplasmic process of the stromal cell, while no or little reaction was detected on the spiral prominence epithelium. In the dark cells of vestibular labyrinth the reaction products were observed on the basolateral interdigitation of the plasmalemma. In contrast, no reaction was observed on the apical cell surface. K+-NPPase activity was most intense in the strial marginal cell, followed by the dark cell of the ampulla and the utricle. The present results revealed that the dark cells in the vestibular labyrinth are involved in endolymph homeostasis.
采用超微细胞化学方法，研究了松鼠猴内耳中钠钾ATP酶复合体对哇巴因敏感的、依赖钾离子的对硝基苯磷酸酶（K⁺-NPPase）活性的定位。在血管纹中，显示K⁺-NPPase活性的反应产物局限于边缘细胞质膜内褶的胞质侧。在螺旋隆起处，基质细胞的胞质突起上也发现了微弱反应，而在螺旋隆起上皮上未检测到反应或仅有少量反应。在前庭迷路的暗细胞中，反应产物出现在质膜的基底外侧指状交错处。相反，在细胞顶端表面未观察到反应。K⁺-NPPase活性在血管纹边缘细胞中最强，其次是壶腹和椭圆囊的暗细胞。目前的结果表明，前庭迷路中的暗细胞参与内淋巴稳态。
