[骨髓增生异常综合征患者骨髓中Th细胞亚群的研究]

[Study of Th cell subsets in bone marrow of myelodysplastic syndromes patients].

作者信息

Wang Xiu-li, Shao Zong-hong, Yao Cheng, He Guang-sheng, Liu Hong, Shi Jun, Bai Jie, Cao Yan-ran, Tu Mei-feng, Wang Hua-quan, Xing Li-min, Sun Juan, Jia Hai-rong, Yang Chong-li

机构信息

Institute of Hematology and Blood Disease Hospital, CAMS, Tianjin 300020, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2005 Dec;26(12):743-5.

PMID:16620580

Abstract

OBJECTIVE

To study the quantity and ratio of Th1, Th2 cells in the bone marrow of myelodysplastic syndromes (MDS) patients, and to evaluate the correlation between the ratio of the blast cells and the number of the Th1 cells in the bone marrow of MDS patients.

METHODS

By FACS, the quantity and ratio of IFN-gamma producing CD4(+) T cell (Th1) and IL-4 producing CD4(+) T cell (Th2) cells in the bone marrow were detected in 21 MDS patients, 18 normal controls and 13 severe aplastic anemia (SAA) patients respectively. The karyotypes of 18 MDS patients and 15 normal controls were assayed. The correlation between the ratio of the blast cells in the bone marrow and the number of the Th1 cells in the MDS patients were analyzed.

RESULTS

The percentages of Th1 cells, Th2 cells and ratio of Th1/Th2 in the bone marrow of normal controls were (0.48 +/- 0.10)%, (0.24 +/- 0.19)% and 2.31 +/- 0.76 respectively, while those of the MDS patients were (0.36 +/- 0.11)%, (0.76 +/- 0.35)% and 0.51 +/- 0.13. The percentage of Th1 cells of patients with MDS was reduced and the Th1/Th2 ratio was significantly lower than that of normal controls (P < 0.01). Those of the patients with SAA were (4.75 +/- 0.49)%, (0.40 +/- 0.28)% and 26.5 +/- 8.79 respectively, their Th1 cells and Th1/Th2 ratio were markedly higher than those of normal controls (P < 0.01). In all of the 15 normal controls the karyotypes were normal, but that of MDS patients was (50.00 +/- 0.10)%. The lower ratio of the Th1 cells in the bone marrow of the patients with MDS and the AML which progressed from MDS was negatively correlated with the higher percentage of the blast cells (r = -0.563, P < 0.01).

CONCLUSIONS

(1) The immune function of T lymphocytes in MDS is abnormal: the balance between Th1 and Th2 cells is broken. (2) With descending of the number of Th1 cells in the bone marrow of the MDS patients, the disease is progressing to leukemia.

摘要

目的

研究骨髓增生异常综合征（MDS）患者骨髓中Th1、Th2细胞的数量及比例，并评估MDS患者骨髓中原始细胞比例与Th1细胞数量之间的相关性。

方法

采用流式细胞术（FACS）分别检测21例MDS患者、18例正常对照者及13例重型再生障碍性贫血（SAA）患者骨髓中产生γ干扰素的CD4(+)T细胞（Th1）和产生白细胞介素-4的CD4(+)T细胞（Th2）的数量及比例。对18例MDS患者和15例正常对照者进行核型分析。分析MDS患者骨髓中原始细胞比例与Th1细胞数量之间的相关性。

结果

正常对照者骨髓中Th1细胞、Th2细胞的百分比及Th1/Th2比例分别为（0.48±0.10）%、（0.24±0.19）%和2.31±0.76，而MDS患者分别为（0.36±0.11）%、（0.76±0.35）%和0.51±0.13。MDS患者Th1细胞百分比降低，Th1/Th2比例显著低于正常对照者（P<0.01）。SAA患者分别为（4.75±0.49）%、（0.40±0.28）%和26.5±8.79，其Th1细胞及Th1/Th2比例显著高于正常对照者（P<0.01）。15例正常对照者核型均正常，而MDS患者核型异常率为（50.00±0.10）%。MDS患者及由MDS进展而来的急性髓系白血病（AML）患者骨髓中Th1细胞比例较低与原始细胞较高百分比呈负相关（r=-0.563，P<0.01）。