[暴露于1800兆赫兹射频场后大鼠海马中NMDA受体表达的影响]

[Effects of NMDA receptor expression in rat's hippocampus after exposure to 1800 MHz radiofrequency field].

作者信息

Huang Chun-tao, Liu Ping, Wu Hui-xin, Wang Jing-lin, Wu Xi-nan

机构信息

Department of Environment, School of Public Health, Kunming Medical College, Yunnan 650032, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2006 Jan;40(1):21-4.

PMID:16620593

Abstract

OBJECTIVE

To identify the effects of NMDA receptor subunits NR2A and NR2B expression in rat's hippocampus after exposure to 1800 MHz radiofrequency radiation.

METHODS

Four-week old female Wistar rats were randomly divided into four groups, with 12 animals for each. The subjects in two experimental groups had been continuously exposed to 1800 MHz microwave radiation (CW) with respective power density of 0.5 mW/cm(2) and 1.0 mW/cm(2) 12 hours each day for 21 days. Meanwhile, sham-controls were carried out. The brain tissue sections were performed by immunohistochemistry to demonstrate both expressions of NR2A, NR2B immune-activity in the hippocampal CA1, CA3 and DG by using computer-assisted image analysis system.

RESULTS

In NR2A: the expression of 0.5 mW/cm(2) power density group was significantly lower than 0 mW/cm(2) power density group in CA3 [(8.5 +/- 1.5) vs (11.1 +/- 1.8), P < 0.01] and had not been significantly changed in CA1 and DG. The expression of 1.0 mW/cm(2) power density group was significantly lower than 0 mW/cm(2) power density group in CA1 and CA3 [(7.9 +/- 1.6) vs (9.7 +/- 1.5); (8.4 +/- 1.7) vs (11.1 +/- 1.8), respective P < 0.05, P < 0.01] and had not been significantly changed in DG. In NR2B: the expression of 0.5 mW/cm(2) power density group was significantly lower than 0 mW/cm(2) power density group in CA1 and CA3 [(16.4 +/- 1.0) vs (17.8 +/- 1.6); (9.6 +/- 1.9) vs (11.2 +/- 2.1), respective P < 0.05]. The expression of 1.0 mW/cm(2) power density group was significantly lower than 0 mW/cm(2) power density group in CA1, CA3 and DG [(13.1 +/- 2.4) vs (17.8 +/- 1.6); (9.3 +/- 1.4) vs (11.2 +/- 2.1); (7.3 +/- 0.1) vs (8.5 +/- 1.0), respective P < 0.01, P < 0.05, P < 0.05].

CONCLUSION

There were findings of the effects on NMDA receptor subunits in different hippocampus sections after exposure to 1800 MHz radiofrequency radiation.

摘要

目的

探讨1800MHz射频辐射后大鼠海马中N-甲基-D-天冬氨酸（NMDA）受体亚基NR2A和NR2B表达的变化。

方法

将4周龄雌性Wistar大鼠随机分为4组，每组12只。两个实验组的大鼠每天分别以0.5mW/cm²和1.0mW/cm²的功率密度连续暴露于1800MHz微波辐射（连续波）12小时，共21天，同时设假照射对照组。采用免疫组织化学方法制备脑组织切片，并用计算机辅助图像分析系统检测海马CA1、CA3和齿状回（DG）中NR2A、NR2B免疫活性的表达。

结果

在NR2A方面：0.5mW/cm²功率密度组CA3区的表达显著低于0mW/cm²功率密度组[（8.5±1.5）对（11.1±1.8），P<0.01]，而CA1区和DG区无明显变化。1.0mW/cm²功率密度组CA1区和CA3区的表达显著低于0mW/cm²功率密度组[（7.9±1.6）对（9.7±1.5）；（8.4±1.7）对（11.1±1.8），P分别<0.05，P<0.01]，DG区无明显变化。在NR2B方面：0.5mW/cm²功率密度组CA1区和CA3区的表达显著低于0mW/cm²功率密度组[（16.4±1.0）对（17.8±1.6）；（9.6±1.9）对（11.2±2.1），P分别<0.05]。1.0mW/cm²功率密度组CA1区、CA3区和DG区的表达显著低于0mW/cm²功率密度组[（13.1±2.4）对（17.8±1.6）；（9.3±1.4）对（11.2±2.1）；（7.3±0.1）对（8.5±1.0），P分别<0.01，P<0.05，P<0.05]。