Pazienza Valerio, Clément Sophie, Pugnale Paolo, Conzelmann Stéphanie, Pascarella Stéphanie, Mangia Alessandra, Negro Francesco
Division of Clinical Pathology, University Hospitals and University of Geneva, Geneva, Switzerland.
Liver Int. 2009 May;29(5):661-9. doi: 10.1111/j.1478-3231.2008.01866.x. Epub 2008 Sep 18.
The liver disease expression in chronic hepatitis C patients is variable and may partially depend on the sequence of the infecting viral genotype.
To identify some hepatitis C virus (HCV) genotype-specific virus-host interactions potentially leading to clinically significant consequences.
We compared the gene expression profile of Huh-7 cells transiently expressing the core protein of HCV genotype 1b and 3a using microarray technology.
Thirty-two genes were overexpressed in Huh-7 transfected with the HCV genotype 1b core protein and 57 genes in cells transfected with the genotype 3a core protein. On the other hand, we found 20 genes downregulated by core 1b and 31 genes by core 3a. These included genes involved in lipid transport and metabolism, cell cycle, immune response and insulin signalling.
The expression of HCV core proteins of different genotypes leads to a specific gene expression profile. This may account for the variable disease expression associated with HCV infection.
慢性丙型肝炎患者的肝脏疾病表现具有多样性，可能部分取决于感染病毒基因型的序列。
确定一些可能导致具有临床意义后果的丙型肝炎病毒（HCV）基因型特异性病毒-宿主相互作用。
我们使用微阵列技术比较了瞬时表达HCV 1b型和3a型核心蛋白的Huh-7细胞的基因表达谱。
在转染HCV 1b型核心蛋白的Huh-7细胞中有32个基因过度表达，在转染3a型核心蛋白的细胞中有57个基因过度表达。另一方面，我们发现有20个基因被1b型核心蛋白下调，31个基因被3a型核心蛋白下调。这些基因包括参与脂质转运和代谢、细胞周期、免疫反应及胰岛素信号传导的基因。
不同基因型的HCV核心蛋白表达导致特定的基因表达谱。这可能解释了与HCV感染相关的疾病表现的多样性。
