Liu Zi-wen, Zhao Yu-pei, Liao Quan, Wu Yuan-de, Zhu Yu
Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China.
Zhonghua Wai Ke Za Zhi. 2006 Jan 1;44(1):36-9.
To explore the possible mechanism of apoptosis induced by photodynamic therapy (PDT) in human pancreatic cancer cells Capan-1 with 2-butylamino-2-demethoxy-hypocrellin B (BAHB) as photosensitizer.
The localization of BAHB in Capan-1 cells was studied, apoptosis was determined by DNA gel electrophoresis after PDT. The mitochondria membrane potential (DYm) and cytochrome C release were observed by laser scan confocal microscopy and Western blotting.
The low concentration photosensitizer was mainly localized in mitochondria and also in lysosomes when the concentration is high. DNA ladder analysis showed characteristic of apoptosis. The mitochondria membrane potential (DYm) showed a loss of 30% around, after 6 hours by PDT under laser scan confocal microscopy, which is caused by a sudden increase in the permeability of mitochondria membrane accompanied with apoptosis. In Western blotting, cytochrome C release was observed from the mitochondria into the cytoplasm during BAHB-induced apoptosis.
The research suggests that BAHB-induced apoptosis is related to photosensitization of mitochondria.
以2-丁氨基-2-去甲氧基竹红菌素B（BAHB）作为光敏剂，探讨光动力疗法（PDT）诱导人胰腺癌细胞Capan-1凋亡的可能机制。
研究BAHB在Capan-1细胞中的定位，PDT后通过DNA凝胶电泳检测细胞凋亡。采用激光扫描共聚焦显微镜和蛋白质免疫印迹法观察线粒体膜电位（ΔΨm）和细胞色素C的释放情况。
低浓度光敏剂主要定位于线粒体，高浓度时也存在于溶酶体中。DNA梯状分析显示出凋亡特征。激光扫描共聚焦显微镜观察发现，PDT照射6小时后，线粒体膜电位（ΔΨm）约丧失30%，这是由于线粒体膜通透性突然增加并伴随凋亡所致。蛋白质免疫印迹法显示，在BAHB诱导的凋亡过程中，观察到细胞色素C从线粒体释放到细胞质中。
研究表明，BAHB诱导的凋亡与线粒体的光致敏作用有关。
