[成年心力衰竭大鼠心肌细胞增殖与心脏功能的关系]

[Relationship between myocyte proliferation and cardiac function in adult rats with heart failure].

作者信息

Li Hao, He Jian-guo, Liu Qing-hua, Deng Da-jun, Wu Zhen-jun, Guo Wen-yuan, Ding Jing-feng, Cheng Xian-sheng

机构信息

Center of Diagnosis and Treatment for Pulmonary Vascular Diseases, Institute of Cardiovascular Diseases, Fuwai Cardiology Hospital, Peking Medical University, Beijing 100037, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2006 Jan 10;86(2):102-5.

PMID:16620714

Abstract

OBJECTIVE

To confirm whether there is myocytes proliferation in the adult rat with heart failure or not, and to investigate the relationship between myocyte proliferation and heart function.

METHODS

Descending anterior branch of left coronary artery was ligated in 20 adult male SD rats so as to establish an heart failure models. Eight rats were used as controls. Hemodynamic parameters, blood pressure (BP), left ventricle end systolic pressure (LVESP), left ventricle end diastolic pressure (LVEDP), +LVdp/dt(max), and -LVdp/dt(max), were measured 30 days after the coronary occlusion. Based on the results of heart function examination, the heart infarct rats were divided into 2 subgroups: cardiac functional compensation subgroup (8 rats), and cardiac functional decompensation subgroup (6 rats). Then the rats were killed and their hearts were taken out and stained with propidium iodide (PI) and antibody to alpha-sarcomeric actin. Immunohistochemistry was used to detect the proliferation cell nuclear antigen (PCNA). Confocal microscopy was used to observe the mitotic image. Light microscopy was used to observe the PCNA positive rate in the myocardium.

RESULTS

(1) Mitotic images of myocytes could be identified by confocal microscopy in the left ventricle of all rats. (2) PCNA expression was detected in the nuclei of both infarct and normal hearts. The PCNA positive rate of the cardiac functional compensation subgroup was 7.2% +/- 1.4%, significantly higher than that of the control group (2.2% +/- 0.8%, P = 0.648). However, the PCNA positive rate of the cardiac functional decompensation subgroup was 3.0% +/- 1.3%, not significantly different from that of the control group (P = 0.648). (3) The correlation coefficient between PCNA-positivity of cardiomyocytes and +LVdp/dt(max) in the infarct rats were 0.80 (P < 0.01) and the correlation coefficient between PCNA-positivity of cardiomyocytes and -LVdp/dt(max) was -0.76 (P = 0.01).

CONCLUSION

(1) There is myocyte proliferation in the adult rat heart. (2) Myocyte proliferation is positively correlated with heart systolic function, and negatively correlated with heart diastolic function in chronic heart failure.

摘要

目的

证实成年心力衰竭大鼠心肌细胞是否存在增殖，并探讨心肌细胞增殖与心功能的关系。

方法

将20只成年雄性SD大鼠左冠状动脉前降支结扎，建立心力衰竭模型。8只大鼠作为对照。冠状动脉结扎30天后测量血流动力学参数、血压（BP）、左心室收缩末期压力（LVESP）、左心室舒张末期压力（LVEDP）、+LVdp/dt（max）和-LVdp/dt（max）。根据心功能检查结果，将心肌梗死大鼠分为2个亚组：心功能代偿亚组（8只大鼠）和心功能失代偿亚组（6只大鼠）。然后处死大鼠，取出心脏，用碘化丙啶（PI）和抗α-肌动蛋白抗体染色。采用免疫组织化学法检测增殖细胞核抗原（PCNA）。用共聚焦显微镜观察有丝分裂图像。用光镜观察心肌中PCNA阳性率。

结果

（1）共聚焦显微镜可在所有大鼠左心室中识别心肌细胞的有丝分裂图像。（2）在梗死心脏和正常心脏的细胞核中均检测到PCNA表达。心功能代偿亚组的PCNA阳性率为7.2%±1.4%，显著高于对照组（2.2%±0.8%，P = 0.648）。然而，心功能失代偿亚组的PCNA阳性率为3.0%±1.3%，与对照组无显著差异（P = 0.648）。（3）梗死大鼠中心肌细胞PCNA阳性与+LVdp/dt（max）的相关系数为0.80（P < 0.01），心肌细胞PCNA阳性与-LVdp/dt（max）的相关系数为-0.76（P = 0.01）。