Xiong Wen, Shao Chao-peng, Li Xue-mei, Zhou Yi-yan
Shenzhen Institute of Transfution Medicine, Shenzhen Blood Center, Shenzhen 518035, China.
Zhonghua Yi Xue Za Zhi. 2006 Jan 10;86(2):128-32.
To study the expression of RhD protein in Rh blood group Del phenotype.
Peripheral blood samples were collected from 3 voluntary blood donors with different Rh phenotypes: CCD(el)D(el)ee, CcD(el)dee, and CcD(el)D(el)ee, and 4 controls: 2 RhD positive with the phenotypes of CCDDee and CcDDEe, and 2 RhD negative with the phenotypes: Ccddee and ccddee. Reverse transcriptase PCR (RT-PCR) and cDNA sequencing were used to detect the RhD mRNA and the expression of Del protein.
The Del individuals all showed complicated RhD mRNA isolations, including 6 transcripts: isoforms with exons 7-9 spliced, exons 7 and 9 spliced, exons 8 and 9 spliced, exon 9 spliced and 2 long transcripts with exons 8 and 9 spliced or exon 9 spliced, but containing an additional segment of sequence from RHD intron 7 (917-1086, GenBank AB035194), those transcripts all being out of exon 9 and the Del9 transcript being the most similar to the normal RhD mRNA.
Normal RhD mRNA does not exist in the Del blood group that does not code normal RhD protein.
研究RhD蛋白在Rh血型Del表型中的表达情况。
采集3名具有不同Rh表型的自愿献血者的外周血样本，其表型分别为CC D（el）D（el）ee、Cc D（el）dee和Cc D（el）D（el）ee，以及4名对照者的外周血样本，其中2名RhD阳性，表型分别为CCDDee和CcDDEe，2名RhD阴性，表型分别为Ccddee和ccddee。采用逆转录聚合酶链反应（RT-PCR）和cDNA测序检测RhD mRNA及Del蛋白的表达。
Del个体均表现出复杂的RhD mRNA分离情况，包括6种转录本：外显子7-9拼接的异构体、外显子7和9拼接的异构体、外显子8和9拼接的异构体、外显子9拼接的异构体以及2种长转录本，其外显子8和9拼接或外显子9拼接，但包含来自RHD内含子7的一段额外序列（917-1086，GenBank AB035194），这些转录本均缺失外显子9，且Del9转录本与正常RhD mRNA最为相似。
Del血型中不存在编码正常RhD蛋白的正常RhD mRNA。
