用于检测、分型和亚型分析水泡性口炎病毒的酶联免疫吸附测定法的开发与评估

Development and evaluation of an enzyme-linked immunosorbent assay for detection, typing, and subtyping of vesicular stomatitis virus.

作者信息

Alonso A, Martins M A, Gomes M da P, Allende R, Söndahl M S

机构信息

Pan American Foot-and-Mouth Disease Center, Rio de Janeiro, Brazil.

出版信息

J Vet Diagn Invest. 1991 Oct;3(4):287-92. doi: 10.1177/104063879100300403.

DOI:10.1177/104063879100300403

PMID:1662076

Abstract

An indirect sandwich enzyme-linked immunosorbent assay (ELISA) has been used for vesicular stomatitis virus (VSV) typing using sets of monovalent and polyvalent rabbit/guinea pig antisera for identification of VSV types New Jersey (VNJ) and Indiana (VIND). The VIND polyvalent antiserum (VIND-P) detects any strain of the 3 subtypes of the VIND type (VIND-1, VIND-2, and VIND-3) with the same strong reactivity. It is also possible to subtype the VIND strains using VIND-P rabbit antiserum as capture antibody and monovalent VIND-1, VIND-2, or VIND-3 guinea pig antisera as detector. The ELISA proposed has about 10 times more sensitivity and provides 10% more positive results than does the complement fixation 50% (CF50) test when epithelial samples are tested.

摘要

一种间接夹心酶联免疫吸附测定（ELISA）已被用于水泡性口炎病毒（VSV）分型，使用单价和多价兔/豚鼠抗血清来鉴定VSV新泽西型（VNJ）和印第安纳型（VIND）。VIND多价抗血清（VIND-P）能以相同的强反应性检测VIND型3个亚型（VIND-1、VIND-2和VIND-3）的任何毒株。使用VIND-P兔抗血清作为捕获抗体，单价VIND-1、VIND-2或VIND-3豚鼠抗血清作为检测抗体，也可以对VIND毒株进行亚型鉴定。当检测上皮样本时，所提出的ELISA灵敏度比补体结合50%（CF50）试验高约10倍，阳性结果多10%。