Alonso A, Martins M A, Gomes M da P, Allende R, Söndahl M S
Pan American Foot-and-Mouth Disease Center, Rio de Janeiro, Brazil.
J Vet Diagn Invest. 1991 Oct;3(4):287-92. doi: 10.1177/104063879100300403.
An indirect sandwich enzyme-linked immunosorbent assay (ELISA) has been used for vesicular stomatitis virus (VSV) typing using sets of monovalent and polyvalent rabbit/guinea pig antisera for identification of VSV types New Jersey (VNJ) and Indiana (VIND). The VIND polyvalent antiserum (VIND-P) detects any strain of the 3 subtypes of the VIND type (VIND-1, VIND-2, and VIND-3) with the same strong reactivity. It is also possible to subtype the VIND strains using VIND-P rabbit antiserum as capture antibody and monovalent VIND-1, VIND-2, or VIND-3 guinea pig antisera as detector. The ELISA proposed has about 10 times more sensitivity and provides 10% more positive results than does the complement fixation 50% (CF50) test when epithelial samples are tested.
一种间接夹心酶联免疫吸附测定（ELISA）已被用于水泡性口炎病毒（VSV）分型，使用单价和多价兔/豚鼠抗血清来鉴定VSV新泽西型（VNJ）和印第安纳型（VIND）。VIND多价抗血清（VIND-P）能以相同的强反应性检测VIND型3个亚型（VIND-1、VIND-2和VIND-3）的任何毒株。使用VIND-P兔抗血清作为捕获抗体，单价VIND-1、VIND-2或VIND-3豚鼠抗血清作为检测抗体，也可以对VIND毒株进行亚型鉴定。当检测上皮样本时，所提出的ELISA灵敏度比补体结合50%（CF50）试验高约10倍，阳性结果多10%。
