Elkaim René, Obrecht-Pflumio Sophie, Tenenbaum Henri
Parogène, 11 rue Humann, 67085 Strasbourg Cedex, France.
Arch Oral Biol. 2006 Sep;51(9):761-8. doi: 10.1016/j.archoralbio.2006.03.001. Epub 2006 Apr 18.
We investigated early biological events initiated by Porphyromonas gingivalis infection of human osteoblasts, focusing on tyrosine-phosphorylation and the expression of key components in focal adhesion and cell signalling.
Human primary osteoblasts were challenged for 1h with Porphyromonas gingivalis. Tyrosine-phosphorylation of paxillin and focal adhesion kinase (FAK) was examined by Western blotting. Changes in alpha3- and beta1-integrin mRNA expression were quantified by RT-PCR.
Tyrosine-phosphorylation of paxillin was proportional to the size of the Porphyromonas gingivalis inoculum. FAK, a potential kinase for paxillin, was not activated. The amount of alpha3- and beta1-integrins, determined by Western blotting, did not vary significantly, while the corresponding mRNA levels fell significantly when a large bacterial inoculum was used.
These results indicate that Porphyromonas gingivalis infection of osteoblasts in vitro triggers tyrosine-phosphorylation of paxillin but not FAK and modify alpha3- and beta1-integrin mRNA expression. This infection thus appears to have different effects on components with essential roles in focal adhesion (paxillin) and cell signalling (FAK and integrins).
我们研究了牙龈卟啉单胞菌感染人成骨细胞引发的早期生物学事件，重点关注酪氨酸磷酸化以及粘着斑和细胞信号传导关键成分的表达。
用人牙龈卟啉单胞菌对人原代成骨细胞进行1小时的刺激。通过蛋白质印迹法检测桩蛋白和粘着斑激酶（FAK）的酪氨酸磷酸化。通过逆转录聚合酶链反应（RT-PCR）定量α3-和β1-整合素mRNA表达的变化。
桩蛋白的酪氨酸磷酸化与牙龈卟啉单胞菌接种量的大小成正比。FAK作为桩蛋白的潜在激酶未被激活。通过蛋白质印迹法测定的α3-和β1-整合素的量没有显著变化，而当使用大量细菌接种物时，相应的mRNA水平显著下降。
这些结果表明，体外牙龈卟啉单胞菌感染成骨细胞会引发桩蛋白的酪氨酸磷酸化，但不会引发FAK的酪氨酸磷酸化，并会改变α3-和β1-整合素mRNA的表达。因此，这种感染似乎对粘着斑（桩蛋白）和细胞信号传导（FAK和整合素）中起重要作用的成分有不同影响。
