Kanda Shigeru, Kanetake Hiroshi, Miyata Yasuyoshi
Department of Molecular Microbiology and Immunology, Division of Endothelial Cell Biology, Nagasaki, Japan.
Biochem Biophys Res Commun. 2006 Jun 2;344(2):617-22. doi: 10.1016/j.bbrc.2006.03.183. Epub 2006 Apr 6.
The signal transduction pathway involved in hepatocyte growth factor (HGF)-induced capillary morphogenesis of endothelial cells was investigated. HGF-induced capillary morphogenesis of the murine spleen endothelial cell line MSS31 was inhibited by a Src family kinase inhibitor, PP2. Stable expression of kinase-inactive Src in MSS31 cells inhibited HGF-induced activation of Src as well as capillary morphogenesis. The HGF-induced capillary morphogenesis of human umbilical vein endothelial cells was also inhibited by PP2 and was reduced by the downregulation of Src by small interfering RNA. These results suggest that HGF induces capillary morphogenesis of endothelial cells through Src.
研究了肝细胞生长因子（HGF）诱导内皮细胞毛细血管形态发生所涉及的信号转导途径。Src家族激酶抑制剂PP2可抑制HGF诱导的小鼠脾脏内皮细胞系MSS31的毛细血管形态发生。MSS31细胞中激酶失活型Src的稳定表达抑制了HGF诱导的Src激活以及毛细血管形态发生。PP2也可抑制HGF诱导的人脐静脉内皮细胞的毛细血管形态发生，小干扰RNA下调Src可使其降低。这些结果表明，HGF通过Src诱导内皮细胞的毛细血管形态发生。
