Kankavi Orhan, Ata Ayhan, Akif Ciftcioglu M
Department of Biochemistry, Burdur Faculty of Veterinary Medicine, The University of Akdeniz, 15100 Burdur, Turkey.
Theriogenology. 2006 Sep 15;66(5):1057-64. doi: 10.1016/j.theriogenology.2006.02.047. Epub 2006 Apr 18.
The presence of surface-active material in the lung alveolus has been known for several decades as being essential for normal lung function. The host defense and controlling inflammatory processes of the lung are the major functions of SP-A and SP-D. SP-A and SP-D were originally demonstrated in alveolar type II cells, but recent studies have shown extrapulmonary expression of SP-A and SP-D indicating systemic roles of these proteins. Present study describes the presence of SP-A and SP-D in the stallion genital tract, prepuce, prostate, testis, and seminal vesicle using Western blotting and immunohistochemistry. This paper presents the first evidence for the existence of SP-A and SP-D glycoproteins in the stallion genital tract. We examined genital system organs and tissues from stallion and were able to show that surfactant protein A and D reactive with surfactant-specific antibodies were present in the stallion genital tract tissues and organs. On the basis of results, it can be postulated that surfactant proteins in the stallion reproductive tract contribute to the immune surveillance and to active barrier defense mechanism.
几十年来，人们已经知道肺泡中表面活性物质的存在对于正常肺功能至关重要。肺表面活性蛋白A（SP-A）和肺表面活性蛋白D（SP-D）的主要功能是宿主防御和控制肺部的炎症过程。SP-A和SP-D最初在II型肺泡细胞中被发现，但最近的研究表明，SP-A和SP-D在肺外也有表达，这表明这些蛋白质具有全身作用。本研究使用蛋白质免疫印迹法和免疫组织化学方法描述了SP-A和SP-D在种马生殖道、包皮、前列腺、睾丸和精囊中的存在情况。本文首次提供了种马生殖道中存在SP-A和SP-D糖蛋白的证据。我们检查了种马的生殖系统器官和组织，发现与表面活性剂特异性抗体反应的表面活性蛋白A和D存在于种马生殖道组织和器官中。根据这些结果，可以推测种马生殖道中的表面活性蛋白有助于免疫监视和主动屏障防御机制。
