Lagarda M J, García-Llatas G, Farré R
Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Burjassot, Spain.
J Pharm Biomed Anal. 2006 Aug 28;41(5):1486-96. doi: 10.1016/j.jpba.2006.02.052. Epub 2006 Apr 18.
Phytosterols are bioactive compounds, one of their most studied and outstanding properties being their cholesterol-lowering activity. This explains the growing interest in the phytosterol contents of foods as either intrinsic or added components. The different steps (extraction, saponification, clean up, chromatographic determination) of plant sterol determination are reviewed, and emphasis is placed on the methods used to assay different phytosterols in food.
植物甾醇是生物活性化合物,其最受研究且最为突出的特性之一是它们的降胆固醇活性。这解释了人们对食品中作为固有成分或添加成分的植物甾醇含量的兴趣日益浓厚。本文综述了植物甾醇测定的不同步骤(提取、皂化、净化、色谱测定),并重点介绍了用于分析食品中不同植物甾醇的方法。