Conchillo Ana, Cercaci Luisito, Ansorena Diana, Rodriguez-Estrada Maria Teresa, Lercker Giovanni, Astiasarán Iciar
Departamento de Bromatología, Tecnología de Alimentos y Toxicología, Facultad de Farmacia, University of Navarra, C/Irunlarrea s/n, E-31080 Pamplona, Spain.
J Agric Food Chem. 2005 Oct 5;53(20):7844-50. doi: 10.1021/jf050539m.
The content of phytosterol oxidation products (POPs) in enriched and nonenriched commercial spreads was evaluated by thin-layer chromatography-gas chromatography (TLC-GC). Oxides of beta-sitosterol, campesterol, and stigmasterol were produced by thermo-oxidation (7-hydroxy, 7-keto, and epoxy derivatives) and chemical synthesis (triol derivatives), which were then separated and identified by TLC-GC. Their identification was further confirmed by GC-mass spectrometry (GC-MS). The total amounts of phytosterols found were 6.07 and 0.33 g/100 g of sample in phytosterol-enriched and nonenriched spread, respectively, whereas the total POPs contents were 45.60 and 13.31 mg/kg of sample in the enriched and nonenriched products. The main POPs found were the 7-keto derivatives of all phytosterols analyzed; 7-ketositosterol was the most abundant one (14.96 and 5.93 mg/kg of sample in phytosterol-enriched and nonenriched spread). No beta-epoxy and triol derivatives were detected in both types of samples. The enriched spread presented a lower phytosterol oxidation rate (0.07%) than the nonenriched one (0.41%).
采用薄层色谱-气相色谱法（TLC-GC）对强化型和非强化型市售涂抹酱中植物甾醇氧化产物（POPs）的含量进行了评估。通过热氧化（7-羟基、7-酮基和环氧衍生物）和化学合成（三醇衍生物）制备了β-谷甾醇、菜油甾醇和豆甾醇的氧化物，然后通过TLC-GC进行分离和鉴定。通过气相色谱-质谱联用（GC-MS）进一步确认了它们的鉴定结果。在富含植物甾醇的涂抹酱和非富含植物甾醇的涂抹酱中，发现的植物甾醇总量分别为6.07 g/100 g样品和0.33 g/100 g样品，而在强化型和非强化型产品中，POPs的总含量分别为45.60 mg/kg样品和13.31 mg/kg样品。所发现的主要POPs是所有分析植物甾醇的7-酮基衍生物；7-酮基谷甾醇是含量最高的一种（在富含植物甾醇的涂抹酱和非富含植物甾醇的涂抹酱中分别为14.96 mg/kg样品和5.93 mg/kg样品）。在两种类型的样品中均未检测到β-环氧和三醇衍生物。强化型涂抹酱的植物甾醇氧化率（0.07%）低于非强化型涂抹酱（0.41%）。
