Liu Qingjun, Huang Huarong, Cai Hua, Xu Ying, Li Yan, Li Rong, Wang Ping
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
Biosens Bioelectron. 2007 Jan 15;22(6):810-5. doi: 10.1016/j.bios.2006.03.006. Epub 2006 Apr 18.
To investigate the use of embryonic stem cells as biosensor elements, mouse embryoid bodies were cultured on the surface of the light-addressable potentiometric sensor and induce to in vitro differentiate into cardiomyocytes and neurons. Extracellular potentials of the cells were recorded by sensor, to detect stem cells potential applications in drugs screening. The experimental results show that known cardiac stimulants (isoproterenol) and relaxants (carbamylcholine) have characteristic effects on the cardiomyocytes in terms of the changes of beat frequency, amplitude and duration. Thus, the embryonic stem cells potentially represent a renewable cell source for the cell-based biosensors.
为了研究胚胎干细胞作为生物传感器元件的用途，将小鼠胚状体培养在光寻址电位传感器表面，并诱导其在体外分化为心肌细胞和神经元。通过传感器记录细胞的细胞外电位，以检测干细胞在药物筛选中的潜在应用。实验结果表明，已知的心脏兴奋剂（异丙肾上腺素）和松弛剂（氨甲酰胆碱）在心跳频率、幅度和持续时间变化方面对心肌细胞具有特征性影响。因此，胚胎干细胞可能代表了基于细胞的生物传感器的可再生细胞来源。
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