一种适用于水稻叶片蛋白质组学研究的二维电泳方案
[A two-dimensional electrophoresis protocol suitable for proteomic study of rice leaves].
作者信息
Wang Yu-Qi, Peng Xin-Xiang
机构信息
Lab of Molecular Plant Physiology, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
出版信息
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Apr;32(2):252-6.
PMID:16622327
Abstract
A 2-DE (two-dimensional electrophoresis) protocol suited for the separation of proteins from rice leaves was established. Protein extraction, quantitative loading of samples and concentrations of the gel were modified and improved to minimize markedly the interference by leaf pigments and other non-protein substances, thus resulting in satisfactory separation results.
摘要
建立了一种适用于分离水稻叶片蛋白质的二维电泳（2-DE）方法。对蛋白质提取、样品定量上样和凝胶浓度进行了改进和优化，以显著减少叶片色素和其他非蛋白质物质的干扰，从而获得了满意的分离效果。
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