Huelseweh Birgit, Ehricht Ralf, Marschall Hans-Juergen
German Armed Forces Scientific Institute for Protection Technologies-NBC Protection, D-29623 Munster, Germany.
Proteomics. 2006 May;6(10):2972-81. doi: 10.1002/pmic.200500721.
A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich-enzyme-linked immunosorbent assay and signal amplification by streptavidin-poly-horseradish peroxidase. Specific immunoassays for Staphylococcus enterotoxin B, ricin, Venezuelan equine encephalitis virus, St. Louis encephalitis virus, West Nile virus, Yellow fever virus, Orthopox virus species, Francisella tularensis, Yersinia pestis, Brucella melitensis, Burkholderia mallei and Escherichia coli EHEC O157:H7 were developed and optimized. All assays could be completed within 1 to 1 1/2 h and detection levels were demonstrated to be as low as in well established ELISAs. Most interesting, as a result of careful antibody screening and testing, it is currently possible to analyse at least five of the "dirty dozen" agents on one single protein chip in parallel.
已开发出一种蛋白质芯片，可同时检测多种不同的生物战剂。该芯片是为ArrayTube平台开发的，提供了一种廉价且易于操作的技术解决方案，它将微管集成蛋白质芯片与夹心酶联免疫吸附测定的经典程序以及链霉亲和素 - 聚辣根过氧化物酶信号放大相结合。针对葡萄球菌肠毒素B、蓖麻毒素、委内瑞拉马脑炎病毒、圣路易斯脑炎病毒、西尼罗河病毒、黄热病病毒、正痘病毒属、土拉弗朗西斯菌、鼠疫耶尔森菌、羊种布鲁氏菌、鼻疽伯克霍尔德菌和大肠杆菌O157:H7开发并优化了特异性免疫测定。所有测定均可在1至1.5小时内完成，检测水平已证明与成熟的酶联免疫吸附测定法一样低。最有意思的是，经过仔细的抗体筛选和测试，目前有可能在一个蛋白质芯片上同时并行分析至少五种“十二大危害物”。
