Savón Clara, Goyenechea Angel, Valdés Odalys, Aguilar José, González Grehete, Palerm Lídice, Gonzalez Guelsys, Pérez Breña Pilar
Instituto de Medicina Tropical Pedro Kourí, Havana, Cuba.
Arch Med Res. 2006 May;37(4):543-7. doi: 10.1016/j.arcmed.2005.08.007.
Respiratory syncytial virus (RSV) is the leading cause of serious lower tract infections in infants. Comorbid conditions such as chronic diseases and prematurity have been associated with greater severity illness, but virus genotypes and disease severity is still unknown.
Forty selected strains of RSV group A and B from Cuban infants with acute respiratory disease (ARD) over five seasons were studied. Viral RNA was extracted and polymerase chain reaction (PCR) was carried out using direct primers directed to parts of the nucleoprotein (N) and fusion (F) genes, respectively. Amplicons were digested using restriction fragment length polymorphism (RFLP) to define the association between virus and disease severity. Disease severity was assessed as very mild, mild, moderate, and severe.
Three of six known N genotypes were detected. NP4 and NP3 were found more frequently; moreover, it was difficult to establish a relationship between N genotypes and disease severity. Five genotypes in F gene were found: F1, F2, F5, F9 and F11; F9 and F11 were associated with very mild disease, but F1 genotype appears to be associated with moderate to severe disease.
At least five combinations of N and F genotypes circulated in the studied infants in Cuba. Patients with F1NP4 genotype showed moderate to severe disease. Relationship between genotypes and disease severity was established.
呼吸道合胞病毒（RSV）是婴儿严重下呼吸道感染的主要病因。诸如慢性病和早产等合并症与病情更严重相关，但病毒基因型与疾病严重程度仍不清楚。
对来自古巴患急性呼吸道疾病（ARD）的婴儿在五个季节中挑选出的40株A组和B组RSV毒株进行研究。提取病毒RNA，分别使用针对核蛋白（N）和融合蛋白（F）基因部分的直接引物进行聚合酶链反应（PCR）。使用限制性片段长度多态性（RFLP）消化扩增产物以确定病毒与疾病严重程度之间的关联。疾病严重程度评估为非常轻微、轻微、中度和重度。
检测到六种已知N基因型中的三种。NP4和NP3更频繁地被发现；此外，难以确定N基因型与疾病严重程度之间的关系。在F基因中发现了五种基因型：F1、F2、F5、F9和F11；F9和F11与非常轻微的疾病相关，但F1基因型似乎与中度至重度疾病相关。
在古巴所研究的婴儿中至少有五种N和F基因型组合在传播。F1NP4基因型的患者表现出中度至重度疾病。确定了基因型与疾病严重程度之间的关系。
