Ma Xiao-dong, Yan Fang, Ma An-de, Wang Hui-jun
Central Laboratory, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Apr;26(4):406-8, 413.
To investigate the effects of resveratrol on the proliferation, apoptosis, mitochondrial membrane potential and cell morphology of human liver cancer cell line HepG2.
The changes in HepG2 cell growth and proliferation in response to resveratrol treatment were evaluated by MTT assay, and resveratrol-induced apoptosis of HepG2 cells was investigated by flow cytometry. Inverted microscope and electron microscope were employed for observing morphological changes of the treated cells. The whole-cell mitochondrial membrane potential was measured in separate experiments using two fluorimetric probes, rhodamine123 and TMRE, respectively. HepG2 cells treated with rhodamine123 were analyzed by flow cytometry and cells treated with TMRE by confocal microscope.
MTT assay showed that low concentrations of resveratrol produced no significant effect on the growth of HepG2 cells, whereas at high concentrations, resveratrol could obviously inhibit the cell growth in a time- and dose-dependent manner. Resveratrol also induced apoptosis of HepG2 cells, and after a 24-hour treatment, resveratrol caused sharp increment of the mitochondria membrane potential.
Resveratrol is capable of inhibiting the proliferation of HepG2 cells and inducing cell apoptosis by depolarizing mitochondrial membrane potential.
研究白藜芦醇对人肝癌细胞系HepG2增殖、凋亡、线粒体膜电位及细胞形态的影响。
采用MTT法评估白藜芦醇处理后HepG2细胞生长和增殖的变化，通过流式细胞术研究白藜芦醇诱导的HepG2细胞凋亡。使用倒置显微镜和电子显微镜观察处理后细胞的形态变化。在单独的实验中分别使用两种荧光探针罗丹明123和四甲基罗丹明乙酯（TMRE）测量全细胞线粒体膜电位。用流式细胞术分析用罗丹明123处理的HepG2细胞，用共聚焦显微镜分析用TMRE处理的细胞。
MTT法显示，低浓度白藜芦醇对HepG2细胞生长无显著影响，而高浓度时，白藜芦醇能明显以时间和剂量依赖性方式抑制细胞生长。白藜芦醇还诱导HepG2细胞凋亡，处理24小时后，白藜芦醇导致线粒体膜电位急剧升高。
白藜芦醇能够通过使线粒体膜电位去极化来抑制HepG2细胞的增殖并诱导细胞凋亡。
