组胺H1受体刺激巨噬细胞样P388D1细胞引起的细胞内钙增加。

Increase in intracellular calcium induced by stimulating histamine H1 receptors in macrophage-like P388D1 cells.

作者信息

Ohno S, Shirai A, Ueda A, Igarashi T, Ishigatsubo Y, Tani K, Okubo T, Hikawa N, Kawakami T, Takenaka T

机构信息

First Department of Internal Medicine, Yokohama City University, School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Dec 31;181(3):1156-63. doi: 10.1016/0006-291x(91)92060-w.

DOI:10.1016/0006-291x(91)92060-w

PMID:1662494

Abstract

The addition of histamine to macrophage-like P388D1 cells resulted in a dose-dependent increase in intracellular calcium [Ca2+]i measured by fura-2 in single cells. The maximum level of [Ca2+]i was obtained by addition of 1 x 10(-4) M histamine. The increase was primarily due to release from the intracellular store. The addition of an H1 specific antagonist pyrilamine before histamine treatment inhibited the increase reversibly, while an H2 specific antagonist cimetidine had no inhibitory effect. Histamine also resulted in a dose-dependent increase in cGMP but not in cAMP. These data suggest the existence of histamine H1 receptors in these cells and histamine may have some biological effect on the function of macrophages via [Ca2+]i and cGMP as the second messengers.

摘要

将组胺添加到巨噬细胞样P388D1细胞中，通过fura - 2在单细胞中测量，细胞内钙[Ca2+]i呈剂量依赖性增加。通过添加1×10(-4) M组胺可获得[Ca2+]i的最大水平。这种增加主要是由于细胞内储存库的释放。在组胺处理前添加H1特异性拮抗剂吡苄明可可逆地抑制这种增加，而H2特异性拮抗剂西咪替丁则没有抑制作用。组胺还导致cGMP呈剂量依赖性增加，但不影响cAMP。这些数据表明这些细胞中存在组胺H1受体，并且组胺可能通过[Ca2+]i和cGMP作为第二信使对巨噬细胞的功能产生一些生物学效应。