Halperin J A, Cornelius F
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, MA 02115.
Biochim Biophys Acta. 1991 Dec 9;1070(2):497-500. doi: 10.1016/0005-2736(91)90092-m.
In proteoliposomes containing reconstituted shark Na,K-ATPase, inside positive potentials open a cation conductance characterized by a voltage-dependence very similar to that found in mammalian erythrocytes. In both proteoliposomes and erythrocytes, the voltage-activated pathway is inhibited by external oligomycin, which traps the Na,K-ATPase in a Na-occluded E1 form. These results indicate that a cation permeable pathway, activated by inside positive potentials, can be ascribed to the Na-K pump--possibly through interaction with its gating mechanism.
在含有重组鲨鱼钠钾ATP酶的蛋白脂质体中，膜内正电位可开启一种阳离子电导，其电压依赖性与在哺乳动物红细胞中发现的非常相似。在蛋白脂质体和红细胞中，电压激活途径均受到外部寡霉素的抑制，寡霉素会将钠钾ATP酶捕获在钠封闭的E1形式中。这些结果表明，由膜内正电位激活的阳离子通透途径可能归因于钠钾泵——可能是通过与其门控机制相互作用。
