Risek B, Gilula N B
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Development. 1991 Sep;113(1):165-81. doi: 10.1242/dev.113.1.165.
The expression of three different members of the gap junction multigene family, alpha 1 (Cx43), beta 1 (Cx32), and beta 2 (Cx26), was analysed in the rat implantation chamber (a structural unit containing fetal, extraembryonic and maternal components within the pregnant uterus) during mid- and late stages of gestation as well as in the delivering, post-partum and non-pregnant uterus. A differential, spatiotemporal and cell-type-specific regulation of gap junctional coexpression was observed for beta 1 and beta 2 in all epithelia examined (visceral, luminal and glandular), as well as for alpha 1 and beta 2 in decidual cells and keratinocytes of the fetal epidermis. alpha 1 antigen was detected in the mesometrial stroma, mesometrial myometrium, connective tissue, mesothelia of the amnion and visceral yolk sac and in the allantoic mesodermal layer throughout gestation. In addition, expression of alpha 1 in the placental basal zone and trophoblast giant cells coincided with the differentiation of these cells. beta 2 expression was observed prominently in the chorionic villi of the placental labyrinth. The presence of beta 1 and beta 2 in the visceral epithelium (visceral yolk sac = the primary route for embryonic nourishment prior to the formation of the chorioallantoic placenta) and beta 2 in the chorionic villi (placental barrier = the major fetomaternal exchange route) suggests that gap junctions have an important role in fetomaternal communication.
在妊娠中期和晚期以及分娩期、产后和非妊娠子宫中，分析了缝隙连接多基因家族的三个不同成员α1（Cx43）、β1（Cx32）和β2（Cx26）在大鼠着床腔（妊娠子宫内包含胎儿、胚外和母体成分的结构单元）中的表达。在所有检查的上皮组织（内脏、腔面和腺上皮）中，观察到β1和β2缝隙连接共表达存在差异、时空特异性和细胞类型特异性调节，在蜕膜细胞和胎儿表皮角质形成细胞中α1和β2也存在这种调节。在整个妊娠期，α1抗原在子宫系膜基质、子宫系膜肌层、结缔组织、羊膜和内脏卵黄囊间皮以及尿囊中层中均有检测到。此外，α1在胎盘基底层和滋养层巨细胞中的表达与这些细胞的分化一致。β2表达主要在胎盘迷路的绒毛膜绒毛中观察到。β1和β2存在于内脏上皮（内脏卵黄囊 = 绒毛膜尿囊胎盘形成前胚胎营养的主要途径）以及β2存在于绒毛膜绒毛（胎盘屏障 = 主要的母胎交换途径）表明缝隙连接在母胎通讯中起重要作用。
